John Michael Newman

All Publications

• COMPARATIVE PATHOLOGY OF A RAREMN1::PATZ1-FUSION NEUROEPITHELIAL PRIMARY TUMOR AND RECURRENCE DEMONSTRATING MARKED HETEROGENEITY, POTENTIAL CHANGES IN TUMOR IMMUNE COMPOSITION, AND CELL-STATE TRANSITIONS Nasajpour, E., Wei, R., Panovska, D., Xing, L., Dhami, T., Che, C., Rabin, L., Feng, Z., Lyle, G., Beale, H., Kephart, E., Vaske, O., Geraldo, A., Newman, J., Vogel, H., Mahaney, K., Campen, C., Jones, J., Ligon, K., Mueller, S., Petritsch, C. OXFORD UNIV PRESS INC. 2025: v70-v71

  View details for DOI 10.1093/neuonc/noaf201.0281

  View details for Web of Science ID 001612032900003

• Neuropathological findings of very low-density lipoprotein receptor-related cerebellar hypoplasia in a full-term fetus. Journal of neuropathology and experimental neurology Newman, J. M., Vogel, H. 2025

  Abstract

  Mutations in the reelin (RELN) extracellular matrix protein gene are known to cause cortical and cerebellar malformations due to disruption of normal neuroblast migration and localization during fetal neurodevelopment. More recently, mutations in genes encoding transmembrane receptors involved in the recognition of reelin, including very low-density lipoprotein receptor (VLDLR), have been linked to various dysequilibrium and ataxia syndromes. Radiologic findings in cases of VLDLR mutations include cerebellar hypoplasia with marked vermis hypoplasia and cortical simplification without lissencephaly. However, the gross and histologic findings in VLDLR-related cerebellar hypoplasia in humans have yet to be described in the literature. Neuropathologic analysis of a confirmed human case could serve to illuminate unique findings and further elucidate the underlying pathophysiologic mechanism of VLDLR gene mutations. We report the autopsy neuropathological findings in a genetically confirmed third-trimester gestation fetal example.

  View details for DOI 10.1093/jnen/nlaf110

  View details for PubMedID 40974083

• Diffuse astrocytoma, AYA-type, frequently MAPK-altered: report of 45 patients. Acta neuropathologica Singh, O., Dampier, C., Abdullaev, Z., Dazelle, K., Chung, H. J., Conway, K., Camelo-Piragua, S., Neill, S., Brown, D., Nix, J. S., Giannini, C., Macaulay, R., Marker, D., Skaugen, J., Kulich, S., Lee, H., Aboud, O., Pytel, P., Borys, E., Perry, A., Naqib-Osman, L., Fernandes, I. L., Mao, Q., Alashari, M., Thomas, C., Helgager, J., Gubbiotti, M. A., Newman, J., Tiwari, N., Cimino, P. J., Quezado, M., Aldape, K. D., Nasrallah, M. P. 2025; 149 (1): 32

  Abstract

  A putative molecular subtype of IDH-wildtype diffuse glioma with recurrent MAPK pathway alterations has recently been reported. By dimensionality reduction analysis of genome-wide methylation profiling, these tumors form a distinct methylation cluster of gliomas. Characterization of 47 tumors from 45 patients reveals that these gliomas are predominantly supratentorial in young adults, are highly infiltrative, and harbor mitogen-activated protein kinase (MAPK) pathway alterations with high rates of CDKN2A/2B deletion, PDGFRA amplification, MYCN amplification, NF1 variants, and BRAF alterations. The tumors' epigenetics are distinct from other adult and pediatric gliomas in the 2021 World Health Organization (WHO) classification. The histology of the gliomas most often demonstrates high-grade astrocytic features, but can be variable from tumor to tumor, as well as fall into a spectrum of histologic grades. Outcomes show considerable variability based on histologic grade and molecular features, supporting grading within this group of tumors to ensure optimal care choices on an individual patient basis. These unifying epigenetic, sequencing, and infiltrative astrocytic features allow the tumors to be considered diffuse astrocytoma, adolescent, and young adult-type, with MAPK alterations (DAYA).

  View details for DOI 10.1007/s00401-025-02873-8

  View details for PubMedID 40202541

  View details for PubMedCentralID 10946093

• Infection by Tickborne Bacterium Candidatus Midichloria Associated with First Trimester Pregnancy Loss, Tennessee, USA. Emerging infectious diseases Newman, J., Hughes, C., Bloch, K. C., Deveaux, K. J., Allen, S., Truong, T. T., Najafian, B., Moncayo, A. C., Tao, L., Lieberman, J., Correa, H. 2025; 31 (2): 350-354

  Abstract

  A previously healthy 26-year-old woman in middle Tennessee, USA, experienced a first trimester pregnancy loss after multiple tick bites. Histopathology, 16S rRNA sequencing, and electron microscopy examination of the products of conception revealed an infection by a bacterium within the Candidatus Midichloria genus.

  View details for DOI 10.3201/eid3102.240870

  View details for PubMedID 39983692

  View details for PubMedCentralID PMC11845134

• Compartment Syndrome Following Snake Envenomation in the United States: A Scoping Review of the Clinical Literature. The western journal of emergency medicine Newman, J., Therriault, C., White, M. S., Nogee, D., Carpenter, J. E. 2024; 25 (4): 651-660

  Abstract

  Local tissue destruction following envenomation from North American snakes, particularly those within the Crotalinae subfamily, has the potential to progress to compartment syndrome. The pathophysiology of venom-induced compartment syndrome (VICS) is a debated topic and is distinct from trauma/reperfusion-induced compartment syndrome. Heterogeneity exists in the treatment practices of VICS, particularly regarding the decision to progress to fasciotomy. Associations with functional outcomes and evolution in clinical practice since the introduction of Crotalidae polyvalent immune Fab (FabAV) have not been well defined. Our goal was to identify the potential gaps in the literature regarding this phenomenon, as well as illuminate salient themes in the clinical characteristics and treatment practices of VICS.We conducted this systematic scoping-style review using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Records were included if they contained data surrounding the envenomation and hospital course of one or more patients who were envenomated by a snake species native to North America and were diagnosed with compartment syndrome from 1980-2020.We included 19 papers: 10 single- or two-patient case reports encompassing 12 patients, and nine chart reviews providing summary statistics of the included patients. In case reports, the median compartment pressure when reported was 60 millimeters of mercury (interquartile range 55-68), 66% underwent fasciotomy, and functional outcomes varied. Use of antivenom appeared to be more liberal with FabAV than the earlier antivenin Crotalidae polyvalent. Rapid progression of swelling was the most commonly reported symptom. Among the included retrospective chart reviews, important data such as compartment pressures, consistent laboratory values, and snake species was inconsistently reported.Venom-induced compartment syndrome is relatively rare. Existing papers generally describe good outcomes even in the absence of surgical management. Significant gaps in the literature regarding antivenom dosing practices, serial compartment pressure measurements, and functional outcomes highlight the need for prospective studies and consistent standardized reporting.

  View details for DOI 10.5811/westjem.18401

  View details for PubMedID 39028252

  View details for PubMedCentralID PMC11254155

• Characterization of pediatric non-hematopoietic tumor metastases to the central nervous system: A single institution review. Journal of neuropathology and experimental neurology Newman, J., Leelatian, N., Liang, J. 2024; 83 (4): 268-275

  Abstract

  Central nervous system (CNS) metastases represent a small portion of pediatric CNS neoplasms and data surrounding this condition with high morbidity is scarce. Single institutional archival institutional pathology records between 1999 and 2022 were searched for patients over 21 years old and younger with CNS, dura, cranial nerve, CSF, or leptomeningeal metastases; 41 cases were identified. We documented primary tumor types and locations, metastasis locations, types of invasion (direct extension vs distant metastasis), times from imaging or pathologic diagnosis to CNS involvement, and outcomes. Distant metastasis was the most common mechanism of metastasis (n = 32, 78%). Interval times to CNS metastasis varied by both tumor type and primary tumor location. In this cohort, osteosarcoma portended the shortest survival following CNS metastasis. This study highlights the diverse mechanisms and locations of CNS involvement in pediatric CNS metastases and illuminates a need for varied monitoring strategies when considering primary tumor type and anatomic location.

  View details for DOI 10.1093/jnen/nlae012

  View details for PubMedID 38350468

• A qualitative assessment of the perceived acceptability and feasibility of eHARTS, a mobile application for transition readiness assessment for adolescents living with HIV in South Africa. PLOS digital health Fomo, M. F., Newman, J., Sibaya, T., Ndlela, N., Hussen, S., Archary, M., Zanoni, B. C. 2023; 2 (6): e0000272

  Abstract

  South Africa has the highest burden of adolescents living with HIV (ALHIV) in the world. The transition from pediatric to adult centered HIV care is a vulnerable period during which many clinical outcomes of ALHIV suffer. Transition readiness assessments may help ALHIV transition from pediatric to adult care to improve their health outcomes. Here, we evaluated the perceived acceptability and feasibility of a mobile health (mHealth) application, eHARTS, to determine transition readiness for ALHIV in South Africa. We conducted in-depth interviews with adolescents (n = 15) and healthcare providers (n = 15) at three government-supported hospitals in KwaZulu-Natal, South Africa. We used a semi-structured interview guide comprising of open-ended questions based on the unified theory of acceptance and use of technology. We did a thematic analysis of the data using an iterative, team-based coding approach to develop themes that were representative of the participants' perspectives on the acceptability and feasibility of eHARTS. We found that most participants found eHARTS to be acceptable because of its simplicity and lack of stigma. Participants believed eHARTS was feasible as it could easily be administered within a hospital setting and integrated into regular clinic activity without disrupting patient care. Additionally, eHARTS was found to have great utility for adolescents and healthcare providers. Clinicians saw it as a valuable tool to engage adolescents and prepare them for transition. Despite concerns that eHARTS may give adolescents a wrong impression about immediate transition, participants suggested that eHARTS be framed in an empowering way as they prepare for transition to adult care. Our data showed that eHARTS is a simple, mobile transition assessment tool with perceived acceptability and feasibility for use in HIV clinics in South Africa for ALHIV. It is particularly useful for ALHIV and transitioning to adult care as it can help identify gaps in readiness for transition.

  View details for DOI 10.1371/journal.pdig.0000272

  View details for PubMedID 37327198

  View details for PubMedCentralID PMC10275417

• S-100 protein and SOX10-positive breast carcinoma mimicking metastatic melanoma. Journal of cutaneous pathology Newman, J., Brahmbhatt, M., Stoff, B. K., Martinez, A. P. 2020; 47 (12): 1187-1191

  Abstract

  We present a case detailing a 70-year-old female with a history of triple-negative breast carcinoma (TNBC) of the left breast and contralateral stage pT2a nodular melanoma of the right upper arm who underwent sentinel lymph node biopsy of the right axilla demonstrating a metastatic epithelioid tumor that was strongly positive for S-100 protein and SOX10. The tumor cells were negative for HMB-45 and Melan-A and positive for CK7 and other breast markers (GCDFP15, mammaglobin, and GATA3). While concerning for metastatic melanoma based on clinical history and initial immunohistochemistry, tumor morphology and subsequent immunohistochemistry was supportive of metastatic breast adenocarcinoma. This case demonstrates a rare but perilous diagnostic pitfall of triple-negative breast carcinomas that strongly and diffusely express S-100 protein and SOX10 mimicking melanoma.

  View details for DOI 10.1111/cup.13822

  View details for PubMedID 32710508