Ken Sutha

All Publications

• Disability Identity Among Diverse Learners and Employees at an Academic Medical Center. JAMA network open Jerome, B., Fassiotto, M., Altamirano, J., Sutha, K., Maldonado, Y., Poullos, P. 2022; 5 (11): e2241948

  Abstract

  This survey study evaluates representation of persons with disabilities across demographic characteristics at an academic medical center.

  View details for DOI 10.1001/jamanetworkopen.2022.41948

  View details for PubMedID 36355375

• Utilising low-cost, easy-to-use microscopy techniques for early peritonitis infection screening in peritoneal dialysis patients. Scientific reports Buckup, M., Kaneda, J. M., Birk, A. M., Glockner, E., Venook, R., Jain, A., Sharma, S., Wong, C., Sutha, K. 2022; 12 (1): 14046

  Abstract

  Peritoneal dialysis (PD) patients are at high risk for peritonitis, an infection of the peritoneum that affects 13% of PD users annually. Relying on subjective peritonitis symptoms results in delayed treatment, leading to high hospitalisation costs, peritoneal scarring, and premature transition to haemodialysis. We have developed and tested a low-cost, easy-to-use technology that uses microscopy and image analysis to screen for peritonitis across the effluent drain tube. Compared to other technologies, our prototype is made from off-the-shelf, low-cost materials. It can be set up quickly and key stakeholders believe it can improve the overall PD experience. We demonstrate that our prototype classifies infection-indicating and healthy white blood cell levels in clinically collected patient effluent with 94% accuracy. Integration of our technology into PD setups as a screening tool for peritonitis would enable earlier physician notification, allowing for prompt diagnosis and treatment to prevent hospitalisations, reduce scarring, and increase PD longevity. Our findings demonstrate the versatility of microscopy and image analysis for infection screening and are a proof of principle for their future applications in health care.

  View details for DOI 10.1038/s41598-022-18380-9

  View details for PubMedID 35982214

• A LOW-COST IN-LINE DEVICE FOR EARLY SCREENING OF PERITONITIS IN PERITONEAL DIALYSIS PATIENTS Kaneda, J., Birk, A., Buckup, M., Glockner, E., Venook, R., Sutha, K. W B SAUNDERS CO-ELSEVIER INC. 2021: 610

  View details for Web of Science ID 000630901900157

• Challenges, Innovations, and Next Steps in Achieving Financial Neutrality for Living Donors CURRENT TRANSPLANTATION REPORTS Mittelman, M., Bertha, R., Sutha, K. 2020; 7 (3): 215-222

  View details for DOI 10.1007/s40472-020-00291-1

  View details for Web of Science ID 000705322200009

• Organoid-based characterization of patient tumors and microenvironments at single cell resolution Salahudeen, A. A., Zhu, J., Ju, J., Batish, A., Sutha, K., Neal, J. T., Giangarra, V., Montesclaros, L., Sapida, J., Sharifi, O., Lee, J., Zheng, G. X., Wagh, D. A., Coller, J. A., Neal, J. W., Padda, S. K., Sabatti, C., Kuo, C. J. AMER ASSOC CANCER RESEARCH. 2018

  View details for DOI 10.1158/1538-7445.AM2018-987

  View details for Web of Science ID 000468818902486

• Osteogenic embryoid body-derived material induces bone formation in vivo. Scientific reports Sutha, K., Schwartz, Z., Wang, Y., Hyzy, S., Boyan, B. D., McDevitt, T. C. 2015; 5: 9960

  Abstract

  The progressive loss of endogenous regenerative capacity that accompanies mammalian aging has been attributed at least in part to alterations in the extracellular matrix (ECM) composition of adult tissues. Thus, creation of a more regenerative microenvironment, analogous to embryonic morphogenesis, may be achieved via pluripotent embryonic stem cell (ESC) differentiation and derivation of devitalized materials as an alternative to decellularized adult tissues, such as demineralized bone matrix (DBM). Transplantation of devitalized ESC materials represents a novel approach to promote functional tissue regeneration and reduce the inherent batch-to-batch variability of allograft-derived materials. In this study, the osteoinductivity of embryoid body-derived material (EBM) was compared to DBM in a standard in vivo ectopic osteoinduction assay in nude mice. EBM derived from EBs differentiated for 10 days with osteogenic media (+β-glycerophosphate) exhibited similar osteoinductivity to active DBM (osteoinduction score = 2.50 ± 0.27 vs. 2.75 ± 0.16) based on histological scoring, and exceeded inactive DBM (1.13 ± 0.13, p < 0.005). Moreover, EBM stimulated formation of new bone, ossicles, and marrow spaces, similar to active DBM. The potent osteoinductivity of EBM demonstrates that morphogenic factors expressed by ESCs undergoing osteogenic differentiation yield a novel devitalized material capable of stimulating de novo bone formation in vivo.

  View details for DOI 10.1038/srep09960

  View details for PubMedID 25961152

  View details for PubMedCentralID PMC4426716

• Osteogenic differentiation of stem cells alters vitamin D receptor expression. Stem cells and development Olivares-Navarrete, R., Sutha, K., Hyzy, S. L., Hutton, D. L., Schwartz, Z., McDevitt, T., Boyan, B. D. 2012; 21 (10): 1726-35

  Abstract

  Pluripotent and multipotent stem cells adopt an osteoblastic phenotype when cultured in environments that enhance their osteogenic potential. Embryonic stem cells differentiated as embryoid bodies (EBs) in osteogenic medium containing β-glycerophosphate exhibit increased expression of bone markers, indicating that cells are osteoblastic. Interestingly, 1α,25-dihydroxyvitaminD3 (1,25D) enhances the osteogenic phenotype not just in EBs but also in multipotent adult mesenchymal stem cells (MSCs). 1,25D acts on osteoblasts via classical vitamin D receptors (VDR) and via a membrane 1,25D-binding protein [protein disulfide isomerase family A, member 3 (PDIA3)], which activates protein kinase C-signaling. The aims of this study were to determine whether these receptors are regulated during osteogenic differentiation of stem cells and if stem cells and differentiated progeny are responsive to 1,25D. mRNA and protein levels for VDR, PDIA3, and osteoblast-associated proteins were measured in undifferentiated cells and in cells treated with osteogenic medium. Mouse EBs expressed both VDR and PDIA3, but VDR increased as cells underwent osteogenic differentiation. Human MSCs expressed Pdia3 at constant levels throughout differentiation, but VDR increased in cells treated with osteogenic medium. These results suggest that both 1,25D signaling mechanisms are important, with PDIA3 playing a greater role during early events and VDR playing a greater role in later stages of differentiation. Understanding these coordinated events provide a powerful tool to control pluripotent and multipotent stem cell differentiation through induction medium.

  View details for DOI 10.1089/scd.2011.0411

  View details for PubMedID 22034957

  View details for PubMedCentralID PMC3376455