All Publications

  • A Case Study in Making Ocean Education Accessible for Students with Special Needs Current: The Journal of Marine Education Clarke, L., Kast, D. J. 2020; 34 (2): 28-35

    View details for DOI 10.5334/cjme.29

  • Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays JOVE-JOURNAL OF VISUALIZED EXPERIMENTS Tiemann, K., Garri, C., Wang, J., Clarke, L., Kani, K. 2018


    Cancer patients with an aberrant regulation of the protein phosphorylation networks are often treated with the tyrosine kinase inhibitors. Response rates approaching 85% are common. Unfortunately, patients often become refractory to the treatment by altering their signal transduction pathways. An implementation of the expression profiling with microarrays can identify the overall mRNA-level changes, and proteomics can identify the overall changes in protein levels or can identify the proteins involved, but the activity of the signal transduction pathways can only be established by interrogating post-translational modifications of the proteins. As a result, the ability to identify whether a drug treatment is successful or whether resistance arose, or the ability to characterize any alterations in the signaling pathways, is an important clinical challenge. Here, we provide a detailed explanation of antibody arrays as a tool which can identify system-wide alterations in various post-translational modifications (e.g., phosphorylation). One of the advantages of using antibody arrays includes their accessibility (an array does not require either an expert in proteomics or costly equipment) and speed. The availability of arrays targeting a combination of post-translational modifications is the primary limitation. In addition, unbiased approaches (phosphoproteomics) may be more suitable for the novel discovery, whereas antibody arrays are ideal for the most widely characterized targets.

    View details for DOI 10.3791/57779

    View details for Web of Science ID 000456210900034

    View details for PubMedID 30295648

    View details for PubMedCentralID PMC6235240