Doctor of Philosophy, Weizmann Institute Of Science (2017)
Master of Science, Weizmann Institute Of Science (2013)
Bachelor of Science, Ben Gurion University Of The Negev (2011)
Characterizing the distribution of myosin H in the apical complex of conoid protruded and conoid retracted Toxoplasma gondii
CELL PRESS. 2022: 409A
View details for Web of Science ID 000759523002535
Cryo-ET of Toxoplasma parasites gives subnanometer insight into tubulin-based structures.
Proceedings of the National Academy of Sciences of the United States of America
2022; 119 (6)
Tubulin is a conserved protein that polymerizes into different forms of filamentous structures in Toxoplasma gondii, an obligate intracellular parasite in the phylum Apicomplexa. Two key tubulin-containing cytoskeletal components are subpellicular microtubules (SPMTs) and conoid fibrils (CFs). The SPMTs help maintain shape and gliding motility, while the CFs are implicated in invasion. Here, we use cryogenic electron tomography to determine the molecular structures of the SPMTs and CFs in vitrified intact and detergent-extracted parasites. Subvolume densities from detergent-extracted parasites yielded averaged density maps at subnanometer resolutions, and these were related back to their architecture in situ. An intralumenal spiral lines the interior of the 13-protofilament SPMTs, revealing a preferred orientation of these microtubules relative to the parasite's long axis. Each CF is composed of nine tubulin protofilaments that display a comma-shaped cross-section, plus additional associated components. Conoid protrusion, a crucial step in invasion, is associated with an altered pitch of each CF. The use of basic building blocks of protofilaments and different accessory proteins in one organism illustrates the versatility of tubulin to form two distinct types of assemblies, SPMTs and CFs.
View details for DOI 10.1073/pnas.2111661119
View details for PubMedID 35121661
NANOSCALE ELUCIDATION OF THE INVASION APPARATUS OF APICOMPLEXAN PARASITES
AMER SOC TROP MED & HYGIENE. 2019: 620
View details for Web of Science ID 000507364505127