Education & Certifications
M.Tech., Indian Institute of Technology (IIT), Kharagpur, Chemical Engineering (2019)
B.Tech., Indian Institute of Technology (IIT), Kharagpur, Chemical Engineering (2019)
Undergraduate Research Fellow (Khorana Scholar), Johns Hopkins University (May 15, 2018 - July 31, 2018)
Principal Investigator: Trina Schroer
Department of Biology
Baltimore, MD 21218, USA
Undergraduate Research Assistant, Indian Institute of Technology (IIT), Kharagpur (November 10, 2015 - May 15, 2019)
Principal Investigator: Sunando DasGupta
Department of Chemical Engineering
Kharagpur, West Bengal 721302
Undergraduate Summer Research Fellow, Indian Institute of Science (IISc), Bangalore (May 4, 2017 - July 28, 2017)
Principal Investigator: Vaishnavi Ananthanarayanan
BioSystems Sciences and Engineering (BSSE)
Interfacial energy driven distinctive pattern formation during the drying of blood droplets.
Journal of colloid and interface science
2020; 573: 307–16
Dried blood droplet morphology may potentially serve as an alternative biomarker for several patho-physiological conditions. The deviant properties of the red blood cells and the abnormal composition of diseased samples are hypothesized to manifest through unique cell-cell and cell-substrate interactions leading to different morphological patterns. Identifying distinctive morphological trait from a large sample size and proposing confirmatory explanations are necessary to establish the signatory pattern as a potential biomarker to differentiate healthy and diseased samples.Comprehensive experimental investigation was undertaken to identify the signatory dried blood droplet patterns. The corresponding image based analysis was in turn used to differentiate the blood samples with a specific haematological disorder "Thalassaemia" from healthy ones. Relevant theoretical analysis explored the role of cell-surface and cell-cell interactions pertinent to the formation of the distinct dried patterns.The differences observed in the dried blood patterns, specifically the radial crack lengths, were found to eventuate from the differences in the overall interaction energies of the system. A first-generation theoretical analysis, with the mean field approximation, also confirmed similar outcome and justified the role of the different physico-chemical properties of red blood cells in diseased samples resulting in shorter radial cracks.
View details for DOI 10.1016/j.jcis.2020.04.008
View details for PubMedID 32289626
DCTN5 mutant mice reveal a role for dynactin in lens biogenesis
ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2019
View details for Web of Science ID 000488800705227
Analysis of the Distinct Pattern Formation of Globular Proteins in the Presence of Micro- and Nanoparticles.
The journal of physical chemistry. B
2018; 122 (38): 8972–84
Pattern formation during evaporation of biofluids has numerous biomedical applications, e.g., in disease identification. The drying of a bidisperse colloidal droplet involves formation of coffee ring patterns owing to the deposition of constituent particles. In the present study, we examine the distinctly different pattern formations during the drying of a colloidal solution depending on the nature of the constituent proteins. The pattern formations of two oppositely charged proteins, namely HSA and lysozyme, have been studied in the presence of fluorescence polystyrene beads of two different sizes (providing better image contrast for further analysis). The variation of pattern formation has been studied by varying the concentrations of the proteins as well as the particles. Furthermore, using image analysis, the patterns are segmented into different regions for quantification. To explain the variations in the patterns, we delve into the interplay of the interactions, especially the capillary and the DLVO forces (between the particles and the substrate). The developed methodology based on the coffee ring effect may be used to identify individual proteins.
View details for DOI 10.1021/acs.jpcb.8b05325
View details for PubMedID 30185036