Contact

  • Academic
    marinaf1@stanford.edu
    University - Student Department: School of Medicine Position: Graduate, Medicine

Additional Info

  • Mail Code: 5151

All Publications

  • Family Engagement With Pediatric Anesthetic Records: A Qualitative Study of Provider-Guardian Communication and Record Design. Paediatric anaesthesia Wallace, K. J., Douglass, M., Gessner, D., Shi, J., Faragalla, M., Schmiesing, C., Wang, E. Y., Xie, J. 2026

    Abstract

    In recent years, health systems worldwide have increasingly expanded patient and guardian access to clinical records. While adult patient perspectives on anesthetic records have been explored, little is known about how pediatric guardians interpret and engage with their child's anesthetic documentation.To explore how guardians of pediatric patients engage with their child's anesthetic record and to identify elements they perceive as meaningful, confusing, or emotionally impactful.We conducted a qualitative study using semi-structured video conference interviews between April 2023 and August 2024 with guardians of pediatric patients undergoing outpatient surgery under general anesthesia at a quaternary academic pediatric hospital. Twenty English-speaking guardians of children classified as American Society of Anesthesiologists Physical Status I-II and receiving anesthesia via endotracheal tube or supraglottic airway were recruited using a semi-purposive convenience sampling approach. During interviews, guardians reviewed their child's record via screen share and provided real-time feedback. Interviews were audio-recorded, transcribed verbatim, and analyzed using inductive and deductive thematic coding until thematic saturation was reached.Guardians identified several elements of the anesthetic record as meaningful, including medications administered, provider involvement, event timelines, and their child's reactions to anesthesia. However, they reported confusion due to unexplained abbreviations, medical jargon, non-chronological data presentation, unclear visual formatting, and perceived inaccuracies. Interpretation was influenced by guardians' comfort level with health information, emotional state, and considerations unique to pediatric care. Emotional responses ranged from reassurance to anxiety; while some guardians found the record useful for future care planning, others described elements as distressing or difficult to interpret.Guardians face substantial barriers when interpreting pediatric anesthetic records. As patient-accessible records become increasingly common across health systems globally, improving anesthetic record design is essential. Plain-language summaries, visual annotations, and pediatric-specific contextual guidance could improve comprehension, reduce misinterpretation, and facilitate better collaboration between guardians and clinicians during perioperative care.

    View details for DOI 10.1002/pan.70149

    View details for PubMedID 41700752

  • Chemoproteomics Identifies State-Dependent and Proteoform-Selective Caspase-2 Inhibitors. Journal of the American Chemical Society Castellón, J. O., Ofori, S., Burton, N. R., Julio, A. R., Turmon, A. C., Armenta, E., Sandoval, C., Boatner, L. M., Takayoshi, E. E., Faragalla, M., Taylor, C., Zhou, A. L., Tran, K., Shek, J., Yan, T., Desai, H. S., Fregoso, O. I., Damoiseaux, R., Backus, K. M. 2024; 146 (22): 14972-14988

    Abstract

    Caspases are a highly conserved family of cysteine-aspartyl proteases known for their essential roles in regulating apoptosis, inflammation, cell differentiation, and proliferation. Complementary to genetic approaches, small-molecule probes have emerged as useful tools for modulating caspase activity. However, due to the high sequence and structure homology of all 12 human caspases, achieving selectivity remains a central challenge for caspase-directed small-molecule inhibitor development efforts. Here, using mass spectrometry-based chemoproteomics, we first identify a highly reactive noncatalytic cysteine that is unique to caspase-2. By combining both gel-based activity-based protein profiling (ABPP) and a tobacco etch virus (TEV) protease activation assay, we then identify covalent lead compounds that react preferentially with this cysteine and afford a complete blockade of caspase-2 activity. Inhibitory activity is restricted to the zymogen or precursor form of monomeric caspase-2. Focused analogue synthesis combined with chemoproteomic target engagement analysis in cellular lysates and in cells yielded both pan-caspase-reactive molecules and caspase-2 selective lead compounds together with a structurally matched inactive control. Application of this focused set of tool compounds to stratify the functions of the zymogen and partially processed (p32) forms of caspase-2 provide evidence to support that caspase-2-mediated response to DNA damage is largely driven by the partially processed p32 form of the enzyme. More broadly, our study highlights future opportunities for the development of proteoform-selective caspase inhibitors that target nonconserved and noncatalytic cysteine residues.

    View details for DOI 10.1021/jacs.3c12240

    View details for PubMedID 38787738

  • Closing the gap: Identifying barriers and facilitators to receiving caudal analgesia for pediatric patients from primarily Spanish-speaking families SPA-AAP Pediatric Anesthesiology Faragalla, M. H., et al 2024