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I work on projects which value knowledges and practices from local communities to inform our understandings of mental health and inspire community connection with the land and sea.

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All Publications

  • The plant terpenoid carvone is a chemotaxis repellent for C. elegans. microPublication. Biology Ellington, C., Hayden, A., LaGrange, Z., Luccioni, M., Osman, M., Ramlan, L., Vogt, M., Guha, S., Goodman, M., O'Connell, L. 2020; 2020

    View details for DOI 10.17912/micropub.biology.000231

    View details for PubMedID 32550506

  • Enabling community-based metrology for wood-degrading fungi. Fungal biology and biotechnology Perez, R., Luccioni, M., Kamakaka, R., Clamons, S., Gaut, N., Stirling, F., Adamala, K. P., Silver, P. A., Endy, D. 2020; 7: 2

    Abstract

    Background: Lignocellulosic biomass could support a greatly-expanded bioeconomy. Current strategies for using biomass typically rely on single-cell organisms and extensive ancillary equipment to produce precursors for downstream manufacturing processes. Alternative forms of bioproduction based on solid-state fermentation and wood-degrading fungi could enable more direct means of manufacture. However, basic methods for cultivating wood-degrading fungi are often ad hoc and not readily reproducible. Here, we developed standard reference strains, substrates, measurements, and methods sufficient to begin to enable reliable reuse of mycological materials and products in simple laboratory settings.Results: We show that a widely-available and globally-regularized consumer product (Pringles) can support the growth of wood-degrading fungi, and that growth on Pringles-broth can be correlated with growth on media made from a fully-traceable and compositionally characterized substrate (National Institute of Standards and Technology Reference Material 8492 Eastern Cottonwood Whole Biomass Feedstock). We also establish a Relative Extension Unit (REU) framework that is designed to reduce variation in quantification of radial growth measurements. So enabled, we demonstrate that five laboratories were able to compare measurements of wood-fungus performance via a simple radial extension growth rate assay, and that our REU-based approach reduced variation in reported measurements by up to~75%.Conclusions: Reliable reuse of materials, measures, and methods is necessary to enable distributed bioproduction processes that can be adopted at all scales, from local to industrial. Our community-based measurement methods incentivize practitioners to coordinate the reuse of standard materials, methods, strains, and to share information supporting work with wood-degrading fungi.

    View details for DOI 10.1186/s40694-020-00092-2

    View details for PubMedID 32206323