Professional Education


  • Doctor of Philosophy, Shanghai Institute Of Cell Biology (2018)
  • Master of Engineering, Grad Sch Of Chinese Univ Sci & Tech (2015)
  • Bachelor of Medicine, Yangzhou University Medical College (2012)
  • Master, University of Chinese Academy of Sciences, Bioengineering (2015)
  • PhD, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Cell Biology (2018)

Stanford Advisors


All Publications


  • A Humanized Anti-GPC3 Antibody for Immuno-Positron Emission Tomography Imaging of Orthotopic Mouse Model of Patient-Derived Hepatocellular Carcinoma Xenografts. Cancers Natarajan, A., Zhang, H., Ye, W., Huttad, L., Tan, M., Chua, M., Gambhir, S. S., So, S. K. 2021; 13 (16)

    Abstract

    Glypican-3 (GPC3) is an attractive diagnostic marker for hepatocellular carcinoma (HCC). We previously reported the potential of an 89Zr-labeled murine anti-GPC3 antibody (clone 1G12) for immunoPET imaging of HCC in orthotopic patient-derived xenograft (PDX) mouse models. We now humanized the murine antibody by complementarity determining region (CDR) grafting, to allow its clinical translation for human use. The engineered humanized anti-GPC3 antibody, clone H3K3, retained comparable binding affinity and specificity to human GPC3. H3K3 was conjugated with desferrioxamine (Df) and radiolabeled with 89Zr to produce the PET/CT tracer 89Zr-Df-H3K3. When injected into GPC3-expressing orthotopic HCC PDX in NOD SCID Gamma (NSG) mice, 89Zr-Df-H3K3 showed specific high uptake into the orthotopic PDX and minimal, non-specific uptake into the non-tumor bearing liver. Specificity was demonstrated by significantly higher uptake of 89Zr-Df-H3K3 into the non-blocked PDX mice, compared with the blocked PDX mice (which received prior injection of 100 mg of unlabeled H3K3). Region of interest (ROI) analysis showed that the PDX/non-tumor liver ratio was highest (mean ± SD: 3.4 ± 0.31) at 168 h post injection; this ratio was consistent with biodistribution studies at the same time point. Thus, our humanized anti-GPC3 antibody, H3K3, shows encouraging potential for use as an immunoPET tracer for diagnostic imaging of HCC patients.

    View details for DOI 10.3390/cancers13163977

    View details for PubMedID 34439132

  • Impact of postoperative radiotherapy for T3N0M0 esophageal cancer patients: A population-based study. Clinical and translational medicine Ge, Y., Yin, L., Tan, M., Dai, W., Jiang, Y., Chen, L., He, X. 2020: e143

    View details for DOI 10.1002/ctm2.143

    View details for PubMedID 32722868