Single-molecule methylation profiles of cell-free DNA in cancer with nanopore sequencing.
2023; 15 (1): 33
Epigenetic characterization of cell-free DNA (cfDNA) is an emerging approach for detecting and characterizing diseases such as cancer. We developed a strategy using nanopore-based single-molecule sequencing to measure cfDNA methylomes. This approach generated up to 200 million reads for a single cfDNA sample from cancer patients, an order of magnitude improvement over existing nanopore sequencing methods. We developed a single-molecule classifier to determine whether individual reads originated from a tumor or immune cells. Leveraging methylomes of matched tumors and immune cells, we characterized cfDNA methylomes of cancer patients for longitudinal monitoring during treatment.
View details for DOI 10.1186/s13073-023-01178-3
View details for PubMedID 37138315
View details for PubMedCentralID 1283450
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