Mohajeet Balveer Bhuckory

All Publications

• Reversible isomerization of rhodopsin imaged in a living eye with phase-sensitive OCT Zhuo, Y., Li, H., Bhuckory, M., Palanker, D. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2025

  View details for Web of Science ID 001568835200006

• Response of the degenerate retina to electrical stimulation is affected by residual photoreceptors Ly, K., Pham-Howard, D., Bhuckory, M., Goldstein, A., Jensen, N., Palanker, D. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2025

  View details for Web of Science ID 001560059200041

• Scaffold on photovoltaic prosthesis replicates the human subretinal debris layer for preclinical studies in rats Bhuckory, M., Shin, A., Ly, K., Mamchik, V., Jensen, N., Devaud, Q., Pham-Howard, D., Goldstein, A., Palanker, D. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2025

  View details for Web of Science ID 001560059200018

• Retinal thermal deformations measured with phase-sensitive optical coherence tomography in vivo. Light, science & applications Zhuo, Y., Bhuckory, M., Li, H., Hattori, J., Pham-Howard, D., Veysset, D., Ling, T., Palanker, D. 2025; 14 (1): 151

  Abstract

  Controlling the tissue temperature rise during retinal laser therapy is essential for predictable outcomes, especially at non-damaging settings. We demonstrate a method for determining the temperature rise in the retina using phase-sensitive optical coherence tomography (pOCT) in vivo. Measurements based on the thermally induced optical path length changes (ΔOPL) in the retina during a 10-ms laser pulse allow detection of the temperature rise with a precision less than 1 °C, which is sufficient for calibration of the laser power for patient-specific non-damaging therapy. We observed a significant difference in confinement of the retinal deformations between the normal and the degenerate retina: in wild-type rats, thermal deformations are localized between the retinal pigment epithelium (RPE) and the photoreceptors' inner segments (IS), as opposed to a deep penetration of the deformations into the inner retinal layers in the degenerate retina. This implies the presence of a structural component within healthy photoreceptors that dampens the tissue expansion induced by the laser heating of the RPE and pigmented choroid. We hypothesize that the thin and soft cilium connecting the inner and outer segments (IS, OS) of photoreceptors may absorb the deformations of the OS and thereby preclude the tissue expansion further inward. Striking difference in the confinement of the retinal deformations induced by a laser pulse in healthy and degenerate retina may be used as a biomechanical diagnostic tool for the characterization of photoreceptors degeneration.

  View details for DOI 10.1038/s41377-025-01798-x

  View details for PubMedID 40175338

  View details for PubMedCentralID PMC11965573

• Enhancing prosthetic vision by upgrade of a subretinal photovoltaic implant in situ. Nature communications Bhuckory, M. B., Monkongpitukkul, N., Shin, A., Kochnev Goldstein, A., Jensen, N., Shah, S. V., Pham-Howard, D., Butt, E., Dalal, R., Galambos, L., Mathieson, K., Kamins, T., Palanker, D. 2025; 16 (1): 2820

  Abstract

  In patients with atrophic age-related macular degeneration, subretinal photovoltaic implant (PRIMA) provided visual acuity up to 20/440, matching its 100 µm pixels size. Next-generation implants with smaller pixels should significantly improve the acuity. This study in rats evaluates removal of a subretinal implant, replacement with a newer device, and the resulting grating acuity in-vivo. Six weeks after the initial implantation with planar and 3-dimensional devices, the retina was re-detached, and the devices were successfully removed. Histology demonstrated a preserved inner nuclear layer. Re-implantation of new devices into the same location demonstrated retinal re-attachment to a new implant. New devices with 22 µm pixels increased the grating acuity from the 100 µm capability of PRIMA implants to 28 µm, reaching the limit of natural resolution in rats. Reimplanted devices exhibited the same stimulation threshold as for the first implantation of the same implants in a control group. This study demonstrates the feasibility of safely upgrading the subretinal photovoltaic implants to improve prosthetic visual acuity.

  View details for DOI 10.1038/s41467-025-58084-y

  View details for PubMedID 40118873

  View details for PubMedCentralID PMC11928519

• 3D electronic implants in subretinal space: Long-term follow-up in rodents. Biomaterials Bhuckory, M. B., Wang, B. Y., Chen, Z. C., Shin, A., Pham-Howard, D., Shah, S., Monkongpitukkul, N., Galambos, L., Kamins, T., Mathieson, K., Palanker, D. 2024; 311: 122674

  Abstract

  Clinical results with photovoltaic subretinal prosthesis (PRIMA) demonstrated restoration of sight via electrical stimulation of the interneurons in degenerated retina, with resolution matching the 100 μm pixel size. Since scaling the pixels below 75 μm in the current bipolar planar geometry will significantly limit the penetration depth of the electric field and increase stimulation threshold, we explore the possibility of using smaller pixels based on a novel 3-dimensional honeycomb-shaped design. We assessed the long-term biocompatibility and stability of these arrays in rats by investigating the anatomical integration of the retina with flat and 3D implants and response to electrical stimulation over lifetime - up to 32-36 weeks post-implantation in aged rats. With both flat and 3D implants, signals elicited in the visual cortex decreased after the day of implantation by more than 3-fold, and gradually recovered over the next 12-16 weeks. With 25 μm high honeycomb walls, the majority of bipolar cells migrate into the wells, while amacrine and ganglion cells remain above the cavities, which is essential for selective network-mediated stimulation of the retina. Retinal thickness and full-field stimulation threshold with 40 μm-wide honeycomb pixels were comparable to those with planar devices - 0.05 mW/mm2 with 10 ms pulses. However, fewer cells from the inner nuclear layer migrated into the 20 μm-wide wells, and stimulation threshold increased over 12-16 weeks, before stabilizing at about 0.08 mW/mm2. Such threshold is still significantly lower than 1.8 mW/mm2 with a previous design of flat bipolar pixels, confirming the promise of the 3D honeycomb-based approach to high resolution subretinal prosthesis.

  View details for DOI 10.1016/j.biomaterials.2024.122674

  View details for PubMedID 38897028

• On selectivity of neural stimulation with subretinal photovoltaic implants Goldstein, A., Jensen, N., Wang, B., Bhuckory, M., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2024

  View details for Web of Science ID 001313316205328

• Transcriptional and Translational Profiling of Reactive Muller Glia Following Cell-type specific Retinal Injury Ashouri, M., Bhuckory, M., Xia, X., Nahmou, M., Tsien, C., Huie, E., Li, B., Cameron, E., Wang, S., Palanker, D. V., Goldberg, J. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2024

  View details for Web of Science ID 001312227704300

• Mapping the retinal resistivity with electrical impedance tomography for modeling of retinal stimulation Shah, S., Goldstein, A., Chen, Z., Vasireddy, P., Bhuckory, M., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2024

  View details for Web of Science ID 001313316205324

• Replacement of a subretinal prosthesis with a higher-resolution array improves grating acuity in the same animal Bhuckory, M., Monkongpitukkul, N., Shin, A., Goldstein, A., Shah, S., Pham-Howard, D., Dalal, R., Galambos, L., Kamins, T., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2024

  View details for Web of Science ID 001313316205325

• Long-term integration of the retina with 3D implants: structure and function Wang, B., Bhuckory, M., Chen, Z., Shin, A., Galambos, L., Mathieson, K., Kamins, T., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2022

  View details for Web of Science ID 000844401305225

• IFT88 Mediates Cilia-Associated Wound Repair in Retinal Pigment Epithelium Ning, K., Bhuckory, M., Kowal, T., Chen, M., Bansal, R., Palanker, D. V., Vollrath, D., Berbari, N., Mahajan, V. B., Hu, Y., Sun, Y. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2022

  View details for Web of Science ID 000844437004307

• Retinal absorption measurements for laser therapy through interferometric imaging of the thermal expansion Veysset, D., Zhuo, Y., Hattori, J., Bhuckory, M., Pandiyan, V., Sabesan, R., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2022

  View details for Web of Science ID 000844401303047

• 3-dimensional subretinal prosthesis with single-cell resolution Bhuckory, M., Chen, Z., Shin, A., Wang, B., Galambos, L., Kamins, T., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2022

  View details for Web of Science ID 000844437006274

• Retinal integration with a subretinal honeycomb-shaped prosthesis Bhuckory, M., Chen, Z., Wang, B., Huang, T., Galambos, L., Shin, A., Kamins, T., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2021

  View details for Web of Science ID 000690761600452

• Dynamic Transcriptional and Translational Profiling of Reactive Muller Glia Following Retinal Injury Ashouri, M., Bhuckory, M., Nahmou, M., Hay, S., Knasel, C. M., Cameron, E. G., Wang, S., Palanker, D. V., Goldberg, J. L. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2021

  View details for Web of Science ID 000690761100769

• Optically configurable confinement of electric field with photovoltaic retinal prosthesis Chen, Z., Wang, B., Bhuckory, M., Huang, T., Galambos, L., Mathieson, K., Kamins, T., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2021

  View details for Web of Science ID 000690761600406

• Subretinal monopolar photovoltaic arrays provide pixel size-independent stimulation threshold and 40 mu m resolution under spatiotemporal modulation Wang, B., Bhuckory, M., Chen, Z., Huang, T., Galambos, L., Mathieson, K., Kamins, T., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2021

  View details for Web of Science ID 000690761600405

• Titration for selective RPE therapy using a continuous line scanning laser Bhuckory, M., Flores, T., Shao, X., Dalal, R., Palanker, D. V. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2019

  View details for Web of Science ID 000488800700240