Noorsher Ahmed
Postdoctoral Scholar, Bioengineering
Professional Education
-
Doctor of Philosophy, University of California San Diego (2024)
-
Doctor of Philosophy, University of California San Diego, Biomedical Sciences (2024)
-
Masters of Arts, Occidental College, Biophysics (2017)
All Publications
-
Bento: a toolkit for subcellular analysis of spatial transcriptomics data.
Genome biology
2024; 25 (1): 82
Abstract
The spatial organization of molecules in a cell is essential for their functions. While current methods focus on discerning tissue architecture, cell-cell interactions, and spatial expression patterns, they are limited to the multicellular scale. We present Bento, a Python toolkit that takes advantage of single-molecule information to enable spatial analysis at the subcellular scale. Bento ingests molecular coordinates and segmentation boundaries to perform three analyses: defining subcellular domains, annotating localization patterns, and quantifying gene-gene colocalization. We demonstrate MERFISH, seqFISH+, Molecular Cartography, and Xenium datasets. Bento is part of the open-source Scverse ecosystem, enabling integration with other single-cell analysis tools.
View details for DOI 10.1186/s13059-024-03217-7
View details for PubMedID 38566187