Peiyu Quan

All Publications

• Selective regulation of macrophage lipid metabolism via nanomaterials' surface chemistry. Nature communications Wu, J., Bai, X., Yan, L., Baimanov, D., Cong, Y., Quan, P., Cai, R., Guan, Y., Bu, W., Lin, B., Wang, J., Yu, S., Li, S., Chong, Y., Li, Y., Hu, G., Zhao, Y., Chen, C., Wang, L. 2024; 15 (1): 8349

  Abstract

  Understanding the interface between nanomaterials and lipoproteins is crucial for gaining insights into their impact on lipoprotein structure and lipid metabolism. Here, we use graphene oxide (GOs) nanosheets as a controlled carbon nanomaterial model to study how surface properties influence lipoprotein corona formation and show that GOs have strong binding affinity with low-density lipoprotein (LDL). We use advanced techniques including X-ray reflectivity, circular dichroism, and molecular simulations to explore the interfacial interactions between GOs and LDL. Specifically, hydrophobic GOs preferentially associate with LDL's lipid components, whereas hydrophilic GOs tend to bind with apolipoproteins. Furthermore, these GOs distinctly modulate a variety of lipid metabolism pathways, including LDL recognition, uptake, hydrolysis, efflux, and lipid droplet formation. This study underscores the importance of structure analysis at the nano-biomolecule interface, emphasizing how nanomaterials' surface properties critically influence cellular lipid metabolism. These insights will inspire the design and application of future biocompatible nanomaterials and nanomedicines.

  View details for DOI 10.1038/s41467-024-52609-7

  View details for PubMedID 39333092

• In situ x-ray imaging to understand subsurface behavior during continuous wave laser drilling APPLIED PHYSICS LETTERS Gorgannejad, S., Martin, A. A., Chen, B., Nicolino, J. W., Strantza, M., Guss, G. M., Forien, J., Thampy, V., Liu, S., Quan, P., Tassone, C. J., Calta, N. P. 2024; 125 (6)

  View details for DOI 10.1063/5.0207380

  View details for Web of Science ID 001284964500004

• Direct mechanistic connection between acoustic signals and melt pool morphology during laser powder bed fusion APPLIED PHYSICS LETTERS Sun, Y., Gorgannejad, S., Martin, A., Nicolino, J., Strantza, M., Forien, J., Thampy, V., Liu, S., Quan, P., Tassone, C. J., Matthews, M. J., Calta, N. P. 2024; 125 (3)

  View details for DOI 10.1063/5.0205663

  View details for Web of Science ID 001281694300001

• Localized keyhole pore prediction during laser powder bed fusion via multimodal process monitoring and X-ray radiography ADDITIVE MANUFACTURING Gorgannejad, S., Martin, A. A., Nicolino, J. W., Strantza, M., Guss, G. M., Khairallah, S., Forien, J., Thampy, V., Liu, S., Quan, P., Tassone, C. J., Calta, N. P. 2023; 78

  View details for DOI 10.1016/j.addma.2023.103810

  View details for Web of Science ID 001108697700001

• Structural Flexibility of Proteins Dramatically Alters Membrane Stability─A Novel Aspect of Lipid-Protein Interaction. The journal of physical chemistry letters Giri, R. P., Mukhopadhyay, M. K., Sanyal, M. K., Bose, D., Chakrabarti, A., Quan, P., Bu, W., Lin, B. 2022; 13 (49): 11430-11437

  Abstract

  Protein isoforms are structural variants with changes in the overall flexibility predominantly at the tertiary level. For membrane associated proteins, such structural flexibility or rigidity affects membrane stability by playing modulatory roles in lipid-protein interaction. Herein, we investigate the protein chain flexibility mediated changes in the mechanistic behavior of phospholipid model membranes in the presence of two well-known isoforms, erythroid (ER) and nonerythroid (NER) spectrin. We show dramatic alterations of membrane elasticity and stability induced by spectrin in the Langmuir monolayers of phosphatidylocholine (PC) and phosphatidylethanolamine (PE) by a combination of isobaric relaxation, surface pressure-area isotherm, X-ray scattering, and microscopy measurements. The NER spectrin drives all monolayers to possess an approximately equal stability, and that required 25-fold increase and 5-fold decrease of stability in PC and PE monolayers, respectively. The untilting transition of the PC membrane in the presence of NER spectrin observed in X-ray measurements can explain better membrane packing and stability.

  View details for DOI 10.1021/acs.jpclett.2c02971

  View details for PubMedID 36468973

• Structure of polymer-capped gold nanorods binding to model phospholipid monolayers JOURNAL OF PHYSICS-MATERIALS Quan, P., Bu, W., Wang, L., Chen, C., Wu, X., Heffern, C., Lee, K. C., Meron, M., Lin, B. 2021; 4 (3)

  View details for DOI 10.1088/2515-7639/abedcd

  View details for Web of Science ID 000640394000001

• Instrument for in situ hard x-ray nanobeam characterization during epitaxial crystallization and materials transformations. The Review of scientific instruments Marks, S. D., Quan, P., Liu, R., Highland, M. J., Zhou, H., Kuech, T. F., Stephenson, G. B., Evans, P. G. 2021; 92 (2): 023908

  Abstract

  Solid-phase epitaxy (SPE) and other three-dimensional epitaxial crystallization processes pose challenging structural and chemical characterization problems. The concentration of defects, the spatial distribution of elastic strain, and the chemical state of ions each vary with nanoscale characteristic length scales and depend sensitively on the gas environment and elastic boundary conditions during growth. The lateral or three-dimensional propagation of crystalline interfaces in SPE has nanoscale or submicrometer characteristic distances during typical crystallization times. An in situ synchrotron hard x-ray instrument allows these features to be studied during deposition and crystallization using diffraction, resonant scattering, nanobeam and coherent diffraction imaging, and reflectivity. The instrument incorporates a compact deposition system allowing the use of short-working-distance x-ray focusing optics. Layers are deposited using radio-frequency magnetron sputtering and evaporation sources. The deposition system provides control of the gas atmosphere and sample temperature. The sample is positioned using a stable mechanical design to minimize vibration and drift and employs precise translation stages to enable nanobeam experiments. Results of in situ x-ray characterization of the amorphous thin film deposition process for a SrTiO3/BaTiO3 multilayer illustrate implementation of this instrument.

  View details for DOI 10.1063/5.0039196

  View details for PubMedID 33648142

• Correlating Ligand Density with Cellular Uptake of Gold Nanorods Revealed by X-ray Reflectivity. Journal of nanoscience and nanotechnology Quan, P., Bu, W., Lin, B., Jiang, X., Wang, L. 2019; 19 (12): 7557-7563

  Abstract

  Ligands can endow unique physicochemical properties of nanomaterials and also mediate biological effects of nanomaterials. It is unclear whether the ligand density affects cytotoxicity and uptake. Herein, we studied the interaction between poly(diallyldimethylammonium chloride)-coated gold nanorods (PDC-Au) and endothelial cells, in which these PDC-Au possessed a series of ligand densities after the storage for different days. We found that PDC-Au with higher density of ligand did not induce obvious cytotoxicity and damage membrane, but more of them were internalized by cells than those with lower ligand density. A powerfully quantitative method, liquid surface X-ray reflectivity, demonstrated that more gold nanorods can be adsorbed on the phospholipid monolayer for PDC-Au with higher ligand density, which directly correlated to the results of cell uptake. The study emphasized the importance of surface ligand density in the evaluation of biological effects of nanomaterials and suggested a cautious consideration in the ligand stability during the design of nanomaterials and their application. Moreover, according to X-ray reflectivity, interfacial analysis is helpful for the study about the interaction between biological membranes and multiple nanomaterials in future, which provides quantitative and structural information.

  View details for DOI 10.1166/jnn.2019.16749

  View details for PubMedID 31196261

• Stability of Ligands on Nanoparticles Regulating the Integrity of Biological Membranes at the Nano-Lipid Interface. ACS nano Wang, L., Quan, P., Chen, S. H., Bu, W., Li, Y. F., Wu, X., Wu, J., Zhang, L., Zhao, Y., Jiang, X., Lin, B., Zhou, R., Chen, C. 2019; 13 (8): 8680-8693

  Abstract

  When nanoparticles interact with cellular or organelle membranes, the coating ligands are known to affect the integrity of the membranes, which regulate cell death and inflammation. However, the molecular mechanisms of this modulation remain unresolved. Here, we use synchrotron X-ray liquid surface scattering and molecular dynamics simulations to study interface structures between phospholipids and gold nanorods (AuNRs) coated by surfactant and polyelectrolyte. These ligands are two types of widely used surface modification with different self-assembled structures and stabilities on the surface of nanoparticles. We reveal distinct mechanisms of the ligand stability in disrupting membrane integrity. We find that the cationic surfactant ligand cetyltrimethylammonium bromide detaches from the AuNRs and inserts into phospholipids, resulting in reduced membrane thickness by compressing the phospholipids to align with the shorter ligand. Conversely, the cationic polyelectrolyte ligand poly(diallyldimethylammonium chloride) is more stable on AuNRs; although it adsorbs onto the membrane, it does not cause much impairment. The distinct coating ligand interactions with phospholipids are further verified by cellular responses including impaired lysosomal membranes and triggered inflammatory effects in macrophages. Together, the quantitative analysis of interface structures elucidates key bio-nano interactions and highlights the importance of surface ligand stability for safety and rational design of nanoparticles.

  View details for DOI 10.1021/acsnano.9b00114

  View details for PubMedID 31329416

• Suppression of Bragg reflection glitches of a single-crystal diamond anvil cell by a polycapillary half-lens in high-pressure XAFS spectroscopy. Journal of synchrotron radiation Chen, D., Dong, J., Zhang, X., Quan, P., Liang, Y., Hu, T., Liu, J., Wu, X., Zhang, Q., Li, Y. 2013; 20 (Pt 2): 243-8

  Abstract

  In combination with a single-crystal diamond anvil cell (DAC), a polycapillary half-lens (PHL) re-focusing optics has been used to perform high-pressure extended X-ray absorption fine-structure measurements. It is found that a large divergent X-ray beam induced by the PHL leads the Bragg glitches from single-crystal diamond to be broadened significantly and the intensity of the glitches to be reduced strongly so that most of the DAC glitches are efficiently suppressed. The remaining glitches can be easily removed by rotating the DAC by a few degrees with respect to the X-ray beam. Accurate X-ray absorption fine-structure (XAFS) spectra of polycrystalline Ge powder with a glitch-free energy range from -200 to 800 eV relative to the Ge absorption edge are obtained using this method at high pressures up to 23.7 GPa, demonstrating the capability of PHL optics in eliminating the DAC glitches for high-pressure XAFS experiments. This approach brings new possibilities to perform XAFS measurements using a DAC up to ultrahigh pressures.

  View details for DOI 10.1107/S0909049513000265

  View details for PubMedID 23412480