- Fusarium and Lomentospora co-infection in a pediatric patient with acute myelogenous leukemia: When Occam's razor may not always apply. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 2022
Lack of mycothiol and ergothioneine induces different protective mechanisms in Mycobacterium smegmatis.
Biochemistry and biophysics reports
2016; 8: 100-106
Mycobacterium smegmatis contains the low molecular weight thiols, mycothiol (MSH) and ergothioneine (ESH). Examination of transposon mutants disrupted in mshC and egtA, involved in the biosynthesis of MSH and ESH respectively, demonstrated that both mutants were sensitive to oxidative, alkylating, and metal stress. However, the mshC mutant exhibited significantly more protein carbonylation and lipid peroxidation than wildtype, while the egtA mutant had less protein and lipid damage than wildtype. We further show that Ohr, KatN, and AhpC, involved in protection against oxidative stress, are upregulated in the egtA mutant. In the mshC mutant, an Usp and a putative thiol peroxidase are upregulated. In addition, mutants lacking MSH also contained higher levels of Coenzyme F420 as compared to wildtype and two Coenzyme F420 dependent enzymes were found to be upregulated. These results indicate that lack of MSH and ESH result in induction of different mechanisms for protecting against oxidative stress.
View details for DOI 10.1016/j.bbrep.2016.08.006
View details for PubMedID 28220152
View details for PubMedCentralID PMC5315357