Shreyas Gopalakrishnan

All Publications

• Barcoded bulk QTL mapping reveals highly polygenic and epistatic architecture of complex traits in yeast. eLife Nguyen Ba, A. N., Lawrence, K. R., Rego-Costa, A., Gopalakrishnan, S., Temko, D., Michor, F., Desai, M. M. 2022; 11

  Abstract

  Mapping the genetic basis of complex traits is critical to uncovering the biological mechanisms that underlie disease and other phenotypes. Genome-wide association studies (GWAS) in humans and quantitative trait locus (QTL) mapping in model organisms can now explain much of the observed heritability in many traits, allowing us to predict phenotype from genotype. However, constraints on power due to statistical confounders in large GWAS and smaller sample sizes in QTL studies still limit our ability to resolve numerous small-effect variants, map them to causal genes, identify pleiotropic effects across multiple traits, and infer non-additive interactions between loci (epistasis). Here, we introduce barcoded bulk quantitative trait locus (BB-QTL) mapping, which allows us to construct, genotype, and phenotype 100,000 offspring of a budding yeast cross, two orders of magnitude larger than the previous state of the art. We use this panel to map the genetic basis of eighteen complex traits, finding that the genetic architecture of these traits involves hundreds of small-effect loci densely spaced throughout the genome, many with widespread pleiotropic effects across multiple traits. Epistasis plays a central role, with thousands of interactions that provide insight into genetic networks. By dramatically increasing sample size, BB-QTL mapping demonstrates the potential of natural variants in high-powered QTL studies to reveal the highly polygenic, pleiotropic, and epistatic architecture of complex traits.

  View details for DOI 10.7554/eLife.73983

  View details for PubMedID 35147078

  View details for PubMedCentralID PMC8979589

• Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations. eLife Johnson, M. S., Gopalakrishnan, S. n., Goyal, J. n., Dillingham, M. E., Bakerlee, C. W., Humphrey, P. T., Jagdish, T. n., Jerison, E. R., Kosheleva, K. n., Lawrence, K. R., Min, J. n., Moulana, A. n., Phillips, A. M., Piper, J. C., Purkanti, R. n., Rego-Costa, A. n., McDonald, M. J., Nguyen Ba, A. N., Desai, M. M. 2021; 10

  Abstract

  Laboratory experimental evolution provides a window into the details of the evolutionary process. To investigate the consequences of long-term adaptation, we evolved 205 Saccharomyces cerevisiae populations (124 haploid and 81 diploid) for ~10,000,000 generations in three environments. We measured the dynamics of fitness changes over time, finding repeatable patterns of declining adaptability. Sequencing revealed that this phenotypic adaptation is coupled with a steady accumulation of mutations, widespread genetic parallelism, and historical contingency. In contrast to long-term evolution in E. coli, we do not observe long-term coexistence or populations with highly elevated mutation rates. We find that evolution in diploid populations involves both fixation of heterozygous mutations and frequent loss-of-heterozygosity events. Together, these results help distinguish aspects of evolutionary dynamics that are likely to be general features of adaptation across many systems from those that are specific to individual organisms and environmental conditions.

  View details for DOI 10.7554/eLife.63910

  View details for PubMedID 33464204