An integrated single- and two-photon non-diffracting light-sheet microscope
REVIEW OF SCIENTIFIC INSTRUMENTS
2018; 89 (4): 043701
We describe a fluorescence optical microscope with both single-photon and two-photon non-diffracting light-sheet excitations for large volume imaging. With a special design to accommodate two different wavelength ranges (visible: 400-700 nm and near infrared: 800-1200 nm), we combine the line-Bessel sheet (LBS, for single-photon excitation) and the scanning Bessel beam (SBB, for two-photon excitation) light sheet together in a single microscope setup. For a transparent thin sample where the scattering can be ignored, the LBS single-photon excitation is the optimal imaging solution. When the light scattering becomes significant for a deep-cell or deep-tissue imaging, we use SBB light-sheet two-photon excitation with a longer wavelength. We achieved nearly identical lateral/axial resolution of about 350/270 nm for both imagings. This integrated light-sheet microscope may have a wide application for live-cell and live-tissue three-dimensional high-speed imaging.
View details for DOI 10.1063/1.5020154
View details for Web of Science ID 000431139400024
View details for PubMedID 29716382
Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
We demonstrate a simple and efficient method for producing ultrathin Bessel ('non-diffracting') light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm, and high-speed, 4D volume imaging of 20 μm sized live sample at 1 Hz temporal resolution.
View details for DOI 10.1038/srep26159
View details for Web of Science ID 000375992000001
View details for PubMedID 27189786
View details for PubMedCentralID PMC4870613