All Publications


  • An integrated single- and two-photon non-diffracting light-sheet microscope REVIEW OF SCIENTIFIC INSTRUMENTS Lau, S., Chiu, H., Zhao, L., Zhao, T., Loy, M. T., Du, S. 2018; 89 (4): 043701

    Abstract

    We describe a fluorescence optical microscope with both single-photon and two-photon non-diffracting light-sheet excitations for large volume imaging. With a special design to accommodate two different wavelength ranges (visible: 400-700 nm and near infrared: 800-1200 nm), we combine the line-Bessel sheet (LBS, for single-photon excitation) and the scanning Bessel beam (SBB, for two-photon excitation) light sheet together in a single microscope setup. For a transparent thin sample where the scattering can be ignored, the LBS single-photon excitation is the optimal imaging solution. When the light scattering becomes significant for a deep-cell or deep-tissue imaging, we use SBB light-sheet two-photon excitation with a longer wavelength. We achieved nearly identical lateral/axial resolution of about 350/270 nm for both imagings. This integrated light-sheet microscope may have a wide application for live-cell and live-tissue three-dimensional high-speed imaging.

    View details for DOI 10.1063/1.5020154

    View details for Web of Science ID 000431139400024

    View details for PubMedID 29716382

  • Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets SCIENTIFIC REPORTS Zhao, T., Lau, S. C., Wang, Y., Su, Y., Wang, H., Cheng, A., Herrup, K., Ip, N. Y., Du, S., Loy, M. M. 2016; 6

    Abstract

    We demonstrate a simple and efficient method for producing ultrathin Bessel ('non-diffracting') light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm, and high-speed, 4D volume imaging of 20 μm sized live sample at 1 Hz temporal resolution.

    View details for DOI 10.1038/srep26159

    View details for Web of Science ID 000375992000001

    View details for PubMedID 27189786

    View details for PubMedCentralID PMC4870613