Contact

  • Work
    tsantiag@stanford.edu
    University - Staff Department: Psych/General Psychiatry and Psychology (Adult) Position: Temp - Non-Exempt
    University - Affiliate Department: Psych/General Psychiatry and Psychology (Adult)

Additional Info

  • Mail Code: 5718

All Publications

  • Glial Contribution to the Pathogenesis of Post-Operative Delirium Revealed by Multi-omic Analysis of Brain Tissue from Neurosurgery Patients. bioRxiv : the preprint server for biology Ishii, T., Wang, T., Shibata, K., Nishitani, S., Yamanashi, T., Wahba, N. E., Seki, T., Thompson, K. J., Yamanishi, K., Nishiguchi, T., Shimura, A., Aoyama, B., Gorantla, N., Phuong, N. J., Nguyen, H. D., Santiago, T. A., Nishizawa, Y., Nagao, T., Howard, M. A., Kawasaki, H., Hino, K., Ikeda, A., Snyder, M. P., Shinozaki, G. 2025

    Abstract

    Post-operative delirium (POD) is a common complication after surgery especially in elderly patients, characterized by acute disturbances in consciousness and cognition, which negatively impacts long-term outcomes. Effective treatments remain elusive due to the unclear pathophysiology of POD. To address the knowledge gap, we investigated DNA methylation profiles and gene expression changes in brain cells from POD and non-POD patients who underwent brain resection surgery for medication refractory epilepsy. DNA methylation analysis revealed alteration in epigenetic status of immune and inflammation-related genes. Single-nucleus RNA sequencing (snRNAseq) identified POD-specific glial cell alterations, particularly in microglia, where neuroinflammation was strongly enhanced, consistent with epigenetic findings. Astrocytes exhibited changes in synapse-related functions and migration. Furthermore, downstream analysis indicated similarities between POD-associated glial cell states and pathologies such as encephalitis and dementia. Overall, this study-the first multi-omics analysis of brain tissue from POD patients-provides direct evidence of glial cell contributions to POD pathogenesis, and highlights potential therapeutic targets.

    View details for DOI 10.1101/2025.03.13.643155

    View details for PubMedID 40161597

    View details for PubMedCentralID PMC11952519

  • Sleep disorders in patients with obsessive-compulsive disorder: A systematic review of the literature. Journal of sleep research Santiago, T., Simbre, I., DelRosso, L. M. 2024: e14446

    Abstract

    Patients with obsessive-compulsive disorder are presumed to be at higher risk of sleep disorders due to the potential interference that persistent thoughts and compulsions may exert on sleep. Although there are studies on sleep findings in patients with obsessive-compulsive disorder, there are few systematic reviews on the presence of sleep disorders in patients with obsessive-compulsive disorder for adults and children. Preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines were followed to perform a comprehensive search of PubMed and Web of Science using the MeSH terms "obsessive-compulsive disorder" and "sleep wake disorders". The exclusion criteria included publications not in English, studies performed on non-humans, abstracts, reviews, and meta-analyses. After applying the exclusion criteria, 17 studies qualified for inclusion in this systematic review. Nine studies were written about children and eight on adults. In the adult studies, sleep questionnaires, actigraphy and dim light melatonin onset showed delayed circadian rhythm in those with obsessive-compulsive disorder. Several studies showed an increased prevalence of insomnia in adult patients with obsessive-compulsive disorder. Overall, these studies showed sleep-onset and maintenance insomnia, and poor sleep quality. In the paediatric studies, sleep questionnaires revealed that paediatric patients with obsessive-compulsive disorder have increased sleep-related problems, including poor sleep quality, difficulty initiating and maintaining sleep, nightmares and sleepwalking. Two studies using actigraphy demonstrated a decreased total sleep time, increased wake after sleep onset and increased duration of awakening. Studies also showed an improvement in sleep symptoms with cognitive behavioural therapy. The systematic review has shown increased sleep-related problems and poor sleep quality both in adult and paediatric patients with obsessive-compulsive disorder. All patients with obsessive-compulsive disorder should therefore be screened for sleep-related problems to help in the overall outcome of treatment plans.

    View details for DOI 10.1111/jsr.14446

    View details for PubMedID 39740046