Contact

  • Academic
    kruzh@stanford.edu
    University - Student Department: Applied Physics Position: Graduate

Additional Info

  • Mail Code: 4090

All Publications

  • Imaging high-frequency voltage dynamics in multiple neuron classes of behaving mammals. bioRxiv : the preprint server for biology Haziza, S., Chrapkiewicz, R., Zhang, Y., Kruzhilin, V., Li, J., Li, J., Delamare, G., Swanson, R., Buzsáki, G., Kannan, M., Vasan, G., Lin, M. Z., Zeng, H., Daigle, T. L., Schnitzer, M. J. 2024

    Abstract

    Fluorescent genetically encoded voltage indicators report transmembrane potentials of targeted cell-types. However, voltage-imaging instrumentation has lacked the sensitivity to track spontaneous or evoked high-frequency voltage oscillations in neural populations. Here we describe two complementary TEMPO voltage-sensing technologies that capture neural oscillations up to ~100 Hz. Fiber-optic TEMPO achieves ~10-fold greater sensitivity than prior photometry systems, allows hour-long recordings, and monitors two neuron-classes per fiber-optic probe in freely moving mice. With it, we uncovered cross-frequency-coupled theta- and gamma-range oscillations and characterized excitatory-inhibitory neural dynamics during hippocampal ripples and visual cortical processing. The TEMPO mesoscope images voltage activity in two cell-classes across a ~8-mm-wide field-of-view in head-fixed animals. In awake mice, it revealed sensory-evoked excitatory-inhibitory neural interactions and traveling gamma and 3-7 Hz waves in the visual cortex, and previously unreported propagation directions for hippocampal theta and beta waves. These technologies have widespread applications probing diverse oscillations and neuron-type interactions in healthy and diseased brains.

    View details for DOI 10.1101/2024.08.15.607428

    View details for PubMedID 39185175

    View details for PubMedCentralID PMC11343216