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https://orcid.org/0000-0001-5568-3417All Publications
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Glutathione dynamics in subcellular compartments and implications for drug development.
Current opinion in chemical biology
2024; 81: 102505
Abstract
Glutathione (GSH) is a pivotal tripeptide antioxidant essential for maintaining cellular redox homeostasis and regulating diverse cellular processes. Subcellular compartmentalization of GSH underscores its multifaceted roles across various organelles including the cytosol, mitochondria, endoplasmic reticulum, and nucleus, each exhibiting distinct regulatory mechanisms. Perturbations in GSH dynamics contribute to pathophysiological conditions, emphasizing the clinical significance of understanding its intricate regulation. This review consolidates current knowledge on subcellular GSH dynamics, highlighting its implications in drug development, particularly in covalent drug design and antitumor strategies targeting intracellular GSH levels. Challenges and future directions in deciphering subcellular GSH dynamics are discussed, advocating for innovative methodologies to advance our comprehension and facilitate the development of precise therapeutic interventions based on GSH modulation.
View details for DOI 10.1016/j.cbpa.2024.102505
View details for PubMedID 39053236
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Fluorescent Probes and Mass Spectrometry-Based Methods to Quantify Thiols in Biological Systems.
Antioxidants & redox signaling
2021
Abstract
Fluorescent probes and mass spectrometry are the two most popular and complementary methods to quantify thiols in biological systems. In this review, we focus on the widely used and commercially available methods to detect and quantify thiols in living cells and the general approaches applied in mass spectrometry-based thiol quantification. We hope this review can serve as a general guide for redox biologists who are interested in thiol species.
View details for DOI 10.1089/ars.2021.0204
View details for PubMedID 34521263