Albina Ibrayeva

All Publications

• Early stem cell aging in the mature brain CELL STEM CELL Ibrayeva, A., Bay, M., Pu, E., Jorg, D. J., Peng, L., Jun, H., Zhang, N., Aaron, D., Lin, C., Resler, G., Hidalgo, A., Jang, M., Simons, B. D., Bonaguidi, M. A. 2021; 28 (5): 955-+

  Abstract

  Stem cell dysfunction drives many age-related disorders. Identifying mechanisms that initially compromise stem cell behavior represent early targets to promote tissue function later in life. Here, we pinpoint multiple factors that disrupt neural stem cell (NSC) behavior in the adult hippocampus. Clonal tracing showed that NSCs exhibit asynchronous depletion by identifying short-term NSCs (ST-NSCs) and long-term NSCs (LT-NSCs). ST-NSCs divide rapidly to generate neurons and deplete in the young brain. Meanwhile, multipotent LT-NSCs are maintained for months but are pushed out of homeostasis by lengthening quiescence. Single-cell transcriptome analysis of deep NSC quiescence revealed several hallmarks of molecular aging in the mature brain and identified tyrosine-protein kinase Abl1 as an NSC aging factor. Treatment with the Abl inhibitor imatinib increased NSC activation without impairing NSC maintenance in the middle-aged brain. Our study indicates that hippocampal NSCs are particularly vulnerable and adaptable to cellular aging.

  View details for DOI 10.1016/j.stem.2021.03.018

  View details for Web of Science ID 000648283600023

  View details for PubMedID 33848469

  View details for PubMedCentralID PMC10069280

• Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device JOURNAL OF CLINICAL MICROBIOLOGY MacMullan, M. A., Chellamuthu, P., Mades, A., Das, S., Turner, F., Slepnev, V., Ibrayeva, A. 2021; 59 (2)

  Abstract

  Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We have evaluated an antibody detection approach using the OraSure Technologies oral antibody collection device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure test for total antibody detection in oral fluid had comparable sensitivity and specificity to commercially available serum-based ELISAs for SARS-CoV-2 antibody detection while allowing for a more accessible form of specimen collection with the potential for self-collection.

  View details for DOI 10.1128/JCM.02510-20

  View details for Web of Science ID 000610235700038

  View details for PubMedID 33234589

  View details for PubMedCentralID PMC8111129

• ELISA detection of SARS-CoV-2 antibodies in saliva SCIENTIFIC REPORTS MacMullan, M. A., Ibrayeva, A., Trettner, K., Deming, L., Das, S., Tran, F., Moreno, J., Casian, J. G., Chellamuthu, P., Kraft, J., Kozak, K., Turner, F. E., Slepnev, V. I., Le Page, L. M. 2020; 10 (1): 20818

  Abstract

  To facilitate containment of the COVID-19 pandemic currently active in the United States and across the world, options for easy, non-invasive antibody testing are required. Here we have adapted a commercially available, serum-based enzyme-linked immunosorbent assay (ELISA) for use with saliva samples, achieving 84.2% sensitivity and 100% specificity in a set of 149 clinical samples. This strategy will enable widespread, affordable testing for patients who experienced this disease, whilst minimizing exposure risk for healthcare workers.

  View details for DOI 10.1038/s41598-020-77555-4

  View details for Web of Science ID 000609201200017

  View details for PubMedID 33257702

  View details for PubMedCentralID PMC7705674

• The Presence of Neural Stem Cells and Changes in Stem Cell-Like Activity With Age in Mouse Spiral Ganglion Cells <i>In Vivo</i> and <i>In Vitro</i> CLINICAL AND EXPERIMENTAL OTORHINOLARYNGOLOGY Moon, B., Ammothumkandy, A., Zhang, N., Peng, L., Ibrayeva, A., Bay, M., Pratap, A., Park, H., Bonaguidi, M., Lu, W. 2018; 11 (4): 224-232

  Abstract

  Spiral ganglion neurons (SGNs) include potential endogenous progenitor populations for the regeneration of the peripheral auditory system. However, whether these populations are present in adult mice is largely unknown. We examined the presence and characteristics of SGN-neural stem cells (NSCs) in mice as a function of age.The expression of Nestin and Ki67 was examined in sequentially dissected cochlear modiolar tissues from mice of different ages (from postnatal day to 24 weeks) and the sphere-forming populations from the SGNs were isolated and differentiated into different cell types.There were significant decreases in Nestin and Ki67 double-positive mitotic progenitor cells in vivo with increasing mouse age. The SGNs formed spheres exhibiting self-renewing activity and multipotent capacity, which were seen in NSCs and were capable of differentiating into neuron and glial cell types. The SGN spheres derived from mice at an early age (postnatal day or 2 weeks) contained more mitotic stem cells than those from mice at a late age.Our findings showed the presence of self-renewing and proliferative subtypes of SGN-NSCs which might serve as a promising source for the regeneration of auditory neurons even in adult mice.

  View details for DOI 10.21053/ceo.2018.00878

  View details for Web of Science ID 000454983300002

  View details for PubMedID 30309200

  View details for PubMedCentralID PMC6222184

• Molecular epidemiology of <i>Mycobacterium tuberculosis</i> strains circulating in the penitentiary system of Kazakhstan INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE Ibrayeva, A., Kozhamkulov, U., Raiymbek, D., Alenova, A., Igilikova, S., Zholdybayeva, E., Abildaev, T., Momynaliev, K. 2014; 18 (3): 298-301

  Abstract

  A total of 60 Mycobacterium tuberculosis isolates collected from patients in prisons in Kazakhstan and 125 from the civilian sector were examined using mycobacterial interspersed repetitive units-variable number of tandem repeat analysis in 2012. The proportion of tuberculosis strains with unique genotypes isolated from the civilian patients was 50.4%, while that in the prison patients was 31.7%. This difference was statistically significant (χ(2) 4.42, P 0.035), and may reflect a low genetic diversity of M. tuberculosis strains isolated from prison patients. The frequencies of mutations in the rpoB531 and katG315 genes of the M. tuberculosis strains isolated from the civilians and in the penitentiary system were not significantly different (rpoB531: 82.4% vs. 88.3%, and katG315: 98.4% vs. 100%, respectively).

  View details for DOI 10.5588/ijtld.13.0558

  View details for Web of Science ID 000331921700010

  View details for PubMedID 24670565