Hsiang-Hua Hung

All Publications

• A microneedle device for rapid dermal interstitial fluid sampling. Science advances Hung, A. H., Kamat, N. U., Bermudez, A., Boczek, S. M., Garcia-Marqués, F. J., Tan, Y. L., Hwang, J. L., Sinawang, P. D., Ilyin, D., Jacobson, G. B., Demirci, U., Poplack, S. P., Pitteri, S. J., DeSimone, J. M. 2025; 11 (39): eadx5492

  Abstract

  Dermal interstitial fluid (ISF) offers a promising alternative to invasive blood tests and opportunities for skin diagnostics. Progress in both the understanding and adoption of ISF tests is hindered by sampling challenges, including lengthy collection times, non-negligible failure rates, variable collection volumes, and inconsistent bioanalyte levels. The causes of many of these issues are not well understood. We demonstrate a microneedle device that is several times faster than state of the art, collecting an average of 15.5 mg of ISF in 5 minutes in humans with near-zero failure rate. This improvement was achieved by designing the spatial pressure gradient driving ISF flow. The influence of penetration depth, collection time, pressure, and age on ISF collection was elucidated, with Darcy's law explaining multiple observations. A data-driven acceptance criterion of <1% blood contamination for ISF is proposed. The device and findings presented will empower researchers to better conduct robust studies in the development of ISF diagnostics.

  View details for DOI 10.1126/sciadv.adx5492

  View details for PubMedID 40991687

  View details for PubMedCentralID PMC12459406

• 3D-Printed Latticed Microneedle Array Patches for Tunable and Versatile Intradermal Delivery. Advanced materials (Deerfield Beach, Fla.) Rajesh, N. U., Luna Hwang, J., Xu, Y., Saccone, M. A., Hung, A. H., Hernandez, R. A., Coates, I. A., Driskill, M. M., Dulay, M. T., Jacobson, G. B., Tian, S., Perry, J. L., DeSimone, J. M. 2024: e2404606

  Abstract

  Using high-resolution 3D printing, a novel class of microneedle array patches (MAPs) is introduced, called latticed MAPs (L-MAPs). Unlike most MAPs which are composed of either solid structures or hollow needles, L-MAPs incorporate tapered struts that form hollow cells capable of trapping liquid droplets. The lattice structures can also be coated with traditional viscous coating formulations, enabling both liquid- and solid-state cargo delivery, on a single patch. Here, a library of 43 L-MAP designs is generated and in-silico modeling is used to down-select optimal geometries for further characterization. Compared to traditionally molded and solid-coated MAPs, L-MAPs can load more cargo with fewer needles per patch, enhancing cargo loading and drug delivery capabilities. Further, L-MAP cargo release kinetics into the skin can be tuned based on formulation and needle geometry. In this work, the utility of L-MAPs as a platform is demonstrated for the delivery of small molecules, mRNA lipid nanoparticles, and solid-state ovalbumin protein. In addition, the production of programmable L-MAPs is demonstrated with tunable cargo release profiles, enabled by combining needle geometries on a single patch.

  View details for DOI 10.1002/adma.202404606

  View details for PubMedID 39221508