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All Publications

  • Biodegradable Citrate-Based Polymers Enable 5D Monitoring of Implant Evolution ADVANCED FUNCTIONAL MATERIALS Shan, D., Wang, D., Ma, Y., Liang, Z., Ravnic, D. J., Zhang, N., Yang, J. 2024

    View details for DOI 10.1002/adfm.202414400

    View details for Web of Science ID 001343913700001

  • Conductive gradient hydrogels allow spatial control of adult stem cell fate. Journal of materials chemistry. B Song, S., McConnell, K. W., Shan, D., Chen, C., Oh, B., Sun, J., Poon, A. S., George, P. M. 2024

    Abstract

    Electrical gradients are fundamental to physiological processes including cell migration, tissue formation, organ development, and response to injury and regeneration. Current electrical modulation of cells is primarily studied under a uniform electrical field. Here we demonstrate the fabrication of conductive gradient hydrogels (CGGs) that display mechanical properties and varying local electrical gradients mimicking physiological conditions. The electrically-stimulated CGGs enhanced human mesenchymal stem cell (hMSC) viability and attachment. Cells on CGGs under electrical stimulation showed a high expression of neural progenitor markers such as Nestin, GFAP, and Sox2. More importantly, CGGs showed cell differentiation toward oligodendrocyte lineage (Oligo2) in the center of the scaffold where the electric field was uniform with a greater intensity, while cells preferred neuronal lineage (NeuN) on the edge of the scaffold on a varying electric field at lower magnitude. Our data suggest that CGGs can serve as a useful platform to study the effects of electrical gradients on stem cells and potentially provide insights on developing new neural engineering applications.

    View details for DOI 10.1039/d3tb02269b

    View details for PubMedID 38291979

  • Culture-Independent Multiplexed Detection of Drug-Resistant Bacteria Using Surface-Enhanced Raman Scattering. ACS sensors Dai, T., Xiao, Z., Shan, D., Moreno, A., Li, H., Prakash, M., Banaei, N., Rao, J. 2023

    Abstract

    The rapid and accurate detection of bacteria resistance to β-lactam antibiotics is critical to inform optimal treatment and prevent overprescription of potent antibiotics. Here, we present a fast, culture-independent method for the detection of extended-spectrum β-lactamases (ESBLs) using surface-enhanced Raman scattering (SERS). The method uses Raman probes that release sulfur-based Raman active molecules in the presence of β-lactamases. The released thiol molecules can be captured by gold nanoparticles, leading to amplified Raman signals. A broad-spectrum cephalosporin probe R1G and an ESBL-specific probe R3G are designed to enable duplex detection of bacteria expressing broad-spectrum β-lactamases or ESBLs with a detection limit of 103 cfu/mL in 1 h incubation. Combined with a portable Raman microscope, our culturing-free SERS assay has reduced screening time to 1.5 h without compromising sensitivity and specificity.

    View details for DOI 10.1021/acssensors.3c01345

    View details for PubMedID 37506677

  • Controlling the Stem Cell Environment Via Conducting Polymer Hydrogels to Enhance Therapeutic Potential ADVANCED MATERIALS TECHNOLOGIES Santhanam, S., Feig, V. R., McConnell, K. W., Song, S., Gardner, E. E., Patel, J. J., Shan, D., Bao, Z., George, P. M. 2023

    View details for DOI 10.1002/admt.202201724

    View details for Web of Science ID 000940740700001