Ingeniero, Universidad De Chile (2013)
Licenciado, Universidad De Chile (2018)
Doctor of Philosophy, University of Manchester (2018)
Judith Frydman, Postdoctoral Faculty Sponsor
CoFe granules focus coordinated glycolytic mRNA localization and translation to fuel glucose fermentation
View details for DOI 10.1101/741231
Translation factor mRNA granules direct protein synthetic capacity to regions of polarized growth.
The Journal of cell biology
mRNA localization serves key functions in localized protein production, making it critical that the translation machinery itself is present at these locations. Here we show that translation factor mRNAs are localized to distinct granules within yeast cells. In contrast to many messenger RNP granules, such as processing bodies and stress granules, which contain translationally repressed mRNAs, these granules harbor translated mRNAs under active growth conditions. The granules require Pab1p for their integrity and are inherited by developing daughter cells in a She2p/She3p-dependent manner. These results point to a model where roughly half the mRNA for certain translation factors is specifically directed in granules or translation factories toward the tip of the developing daughter cell, where protein synthesis is most heavily required, which has particular implications for filamentous forms of growth. Such a feedforward mechanism would ensure adequate provision of the translation machinery where it is to be needed most over the coming growth cycle.
View details for DOI 10.1083/jcb.201704019
View details for PubMedID 30877141