Bio


Dr. Jingxun Chen obtained her Ph.D. in Molecular and Cell Biology from the University of California, Berkeley. She studied the fundamental mechanisms of gene expression, protein degradation, and chromosome segregation. She then joined Dr. Anne Brunet's lab at Stanford University. Her current research focuses on understanding how sexual interaction affects vertebrate organ aging, using the African turquoise killifish as a model. Her long-term research goals are to 1) uncover the causative roles of specific molecular players (e.g., genes, cell types, circulating factors) in mediating the effects of sexual interaction on organ aging and 2) assess the resilience of sexually active animals responding to stress/insults, such as organ injury and different diets.

Honors & Awards


  • FASEB Reproductive Aging Meeting Poster Award, FASEB Meeting (2022)
  • Stanford Postdoc Justice Equity Diversity and Inclusion Champion Award, Stanford Office of Postdoctoral Affairs (2022)
  • Stanford Jump Start Award, Stanford University (2021)
  • Jane Coffin Childs Fellow, Jane Coffin Childs Fund for Medical Research (2020)
  • UC Berkeley Cris Alvaro Memorial Prize, UC Berkeley (2019)
  • Outstanding Graduate Student Instructor Award, UC Berkeley (2018)
  • Teaching Effectiveness Award, UC Berkeley (2018)
  • National Science Foundation Graduate Research Fellowship, National Science Foundation (2015)
  • Genetics, Genomics & Development Division Poster Award, UC Berkeley (2013)
  • Patrick and Judy Young Fellowship, UC Berkeley (2013)
  • ABRCMS Poster Award in the Cell Biology Division, Annual Biomedical Research Conference for Minority Study (ABRCMS) (2011)
  • ABRCMS Travel Award, Annual Biomedical Research Conference for Minority Study (ABRCMS) (2011)
  • Amgen Scholar, UC Berkeley (2011)
  • Lord Foundation Undergraduate Research Opportunities Program Funding, MIT (2010)
  • National Science and Mathematics Access to Retain Talent Grant Awardee, US Department of Education (2010)
  • Public Service Grant Awardee, MIT (2010)
  • Bay Area Engineering Council Scholarship Award, Bay Area Engineering Council (2009)
  • MIT Club of Northern California Judy and Phil Friend '58 Memorial Scholar, MIT (2009)

Boards, Advisory Committees, Professional Organizations


  • Coordinator, Stanford Someone Like Me Program (2021 - Present)

Professional Education


  • Ph.D., University of California, Berkeley, Molecular and Cell Biology (2019)
  • B.S., Massachusetts Institute of Technology, Biology (2013)

Stanford Advisors


Current Research and Scholarly Interests


Understand the molecular impact of sexual interaction on vertebrate organ aging

Lab Affiliations


All Publications


  • Age-related changes in the zebrafish and killifish inner ear and lateral line. Scientific reports Coffin, A. B., Dale, E., Molano, O., Pederson, A., Costa, E. K., Chen, J. 2024; 14 (1): 6670

    Abstract

    Age-related hearing loss (ARHL) is a debilitating disorder for millions worldwide. While there are multiple underlying causes of ARHL, one common factor is loss of sensory hair cells. In mammals, new hair cells are not produced postnatally and do not regenerate after damage, leading to permanent hearing impairment. By contrast, fish produce hair cells throughout life and robustly regenerate these cells after toxic insult. Despite these regenerative abilities, zebrafish show features of ARHL. Here, we show that aged zebrafish of both sexes exhibited significant hair cell loss and decreased cell proliferation in all inner ear epithelia (saccule, lagena, utricle). Ears from aged zebrafish had increased expression of pro-inflammatory genes and significantly more macrophages than ears from young adult animals. Aged zebrafish also had fewer lateral line hair cells and less cell proliferation than young animals, although lateral line hair cells still robustly regenerated following damage. Unlike zebrafish, African turquoise killifish (an emerging aging model) only showed hair cell loss in the saccule of aged males, but both sexes exhibit age-related changes in the lateral line. Our work demonstrates that zebrafish exhibit key features of auditory aging, including hair cell loss and increased inflammation. Further, our finding that aged zebrafish have fewer lateral line hair cells yet retain regenerative capacity, suggests a decoupling of homeostatic hair cell addition from regeneration following acute trauma. Finally, zebrafish and killifish show species-specific strategies for lateral line homeostasis that may inform further comparative research on aging in mechanosensory systems.

    View details for DOI 10.1038/s41598-024-57182-z

    View details for PubMedID 38509148

    View details for PubMedCentralID 7589617

  • The African Turquoise Killifish: A Scalable Vertebrate Model for Aging and Other Complex Phenotypes. Cold Spring Harbor protocols Boos, F., Chen, J., Brunet, A. 2023

    Abstract

    The African turquoise killifish Nothobranchius furzeri is currently the shortest-lived vertebrate that can be bred in captivity. Because of its short life span of only 4-6 months, rapid generation time, high fecundity, and low cost of maintenance, the African turquoise killifish has emerged as an attractive model organism that combines the scalability of invertebrate models with the unique features of vertebrate organisms. A growing community of researchers is using the African turquoise killifish for studies in diverse fields, including aging, organ regeneration, development, "suspended animation," evolution, neuroscience, and disease. A wide range of techniques is now available for killifish research, from genetic manipulations and genomic tools to specialized assays for studying life span, organ biology, response to injury, etc. This protocol collection provides detailed descriptions of the methods that are generally applicable to all killifish laboratories and those that are limited to specific disciplines. Here, we give an overview of the features that render the African turquoise killifish a unique fast-track vertebrate model organism.

    View details for DOI 10.1101/pdb.over107737

    View details for PubMedID 37100468

  • Breeding and Reproduction of the African Turquoise Killifish Nothobranchius furzeri. Cold Spring Harbor protocols Chen, J., Khondker, R. C., Brunet, A. 2023

    Abstract

    The successful breeding and reproduction of the African turquoise killifish Nothobranchius furzeri in a controlled laboratory setting are required to establish this fish species as a model system for studying vertebrate development and aging. Here, we describe a protocol to care for and hatch African turquoise killifish embryos, raise the juvenile fish to adulthood, and breed this species using sand as the breeding bedding. We also provide suggestions for generating a large quantity of good-quality embryos.

    View details for DOI 10.1101/pdb.prot107816

    View details for PubMedID 36863853

  • An automated feeding system for the African killifish reveals effects of dietary restriction on lifespan and allows scalable assessment of associative learning. eLife McKay, A., Costa, E. K., Chen, J., Hu, C., Chen, X., Bedbrook, C. N., Khondker, R. C., Thielvoldt, M., Priya Singh, P., Wyss-Coray, T., Brunet, A. 2022; 11

    Abstract

    The African turquoise killifish is an exciting new vertebrate model for aging studies. A significant challenge for any model organism is the control over its diet in space and time. To address this challenge, we created an automated and networked fish feeding system. Our automated feeder is designed to be open-source, easily transferable, and built from widely available components. Compared to manual feeding, our automated system is highly precise and flexible. As a proof-of-concept for the feeding flexibility of these automated feeders, we define a favorable regimen for growth and fertility for the African killifish and a dietary restriction regimen where both feeding time and quantity are reduced. We show that this dietary restriction regimen extends lifespan in males (but not in females) and impacts the transcriptomes of killifish livers in a sex-specific manner. Moreover, combining our automated feeding system with a video camera, we establish a quantitative associative learning assay to provide an integrative measure of cognitive performance for the killifish. The ability to precisely control food delivery in the killifish opens new areas to assess lifespan and cognitive behavior dynamics and to screen for dietary interventions and drugs in a scalable manner previously impossible with traditional vertebrate model organisms.

    View details for DOI 10.7554/eLife.69008

    View details for PubMedID 36354233

  • Meiotic regulation of the Ndc80 complex composition and function. Current genetics Chen, J., Ünal, E. 2021; 67 (4): 511-518

    Abstract

    This review describes the current models for how the subunit abundance of the Ndc80 complex, a key kinetochore component, is regulated in budding yeast and metazoan meiosis. The past decades of kinetochore research have established the Ndc80 complex to be a key microtubule interactor and a central hub for regulating chromosome segregation. Recent studies further demonstrate that Ndc80 is the limiting kinetochore subunit that dictates the timing of kinetochore activation in budding yeast meiosis. Here, we discuss the molecular circuits that regulate Ndc80 protein synthesis and degradation in budding yeast meiosis and compare the findings with those from metazoans. We envision the regulatory principles discovered in budding yeast to be conserved in metazoans, thereby providing guidance into future investigations on kinetochore regulation in human health and disease.

    View details for DOI 10.1007/s00294-021-01174-3

    View details for PubMedID 33745061

    View details for PubMedCentralID PMC8254699

  • Tunable Transcriptional Interference at the Endogenous Alcohol Dehydrogenase Gene Locus in Drosophila melanogaster. G3 (Bethesda, Md.) Jorgensen, V., Chen, J., Vander Wende, H., Harris, D. E., McCarthy, A., Breznak, S., Wong-Deyrup, S. W., Chen, Y., Rangan, P., Brar, G. A., Sawyer, E. M., Chan, L. Y., Ünal, E. 2020; 10 (5): 1575-1583

    Abstract

    Neighboring sequences of a gene can influence its expression. In the phenomenon known as transcriptional interference, transcription at one region in the genome can repress transcription at a nearby region in cis Transcriptional interference occurs at a number of eukaryotic loci, including the alcohol dehydrogenase (Adh) gene in Drosophila melanogasterAdh is regulated by two promoters, which are distinct in their developmental timing of activation. It has been shown using transgene insertion that when the promoter distal from the Adh start codon is deleted, transcription from the proximal promoter becomes de-regulated. As a result, the Adh proximal promoter, which is normally active only during the early larval stages, becomes abnormally activated in adults. Whether this type of regulation occurs in the endogenous Adh context, however, remains unclear. Here, we employed the CRISPR/Cas9 system to edit the endogenous Adh locus and found that removal of the distal promoter also resulted in the untimely expression of the proximal promoter-driven mRNA isoform in adults, albeit at lower levels than previously reported. Importantly, transcription from the distal promoter was sufficient to repress proximal transcription in larvae, and the degree of this repression was dependent on the degree of distal promoter activity. Finally, upregulation of the distal Adh transcript led to the enrichment of histone 3 lysine 36 trimethylation over the Adh proximal promoter. We conclude that the endogenous Adh locus is developmentally regulated by transcriptional interference in a tunable manner.

    View details for DOI 10.1534/g3.119.400937

    View details for PubMedID 32213532

    View details for PubMedCentralID PMC7202008

  • Aurora B-dependent Ndc80 degradation regulates kinetochore composition in meiosis. Genes & development Chen, J., Liao, A., Powers, E. N., Liao, H., Kohlstaedt, L. A., Evans, R., Holly, R. M., Kim, J. K., Jovanovic, M., Ünal, E. 2020; 34 (3-4): 209-225

    Abstract

    The kinetochore complex is a conserved machinery that connects chromosomes to spindle microtubules. During meiosis, the kinetochore is restructured to accommodate a specialized chromosome segregation pattern. In budding yeast, meiotic kinetochore remodeling is mediated by the temporal changes in the abundance of a single subunit called Ndc80. We previously described the regulatory events that control the timely synthesis of Ndc80. Here, we report that Ndc80 turnover is also tightly regulated in meiosis: Ndc80 degradation is active in meiotic prophase, but not in metaphase I. Ndc80 degradation depends on the ubiquitin ligase APCAma1 and is mediated by the proteasome. Importantly, Aurora B-dependent Ndc80 phosphorylation, a mark that has been previously implicated in correcting erroneous microtubule-kinetochore attachments, is essential for Ndc80 degradation in a microtubule-independent manner. The N terminus of Ndc80, including a 27-residue sequence and Aurora B phosphorylation sites, is both necessary and sufficient for kinetochore protein degradation. Finally, defects in Ndc80 turnover predispose meiotic cells to chromosome mis-segregation. Our study elucidates the mechanism by which meiotic cells modulate their kinetochore composition through regulated Ndc80 degradation, and demonstrates that Aurora B-dependent regulation of kinetochores extends beyond altering microtubule attachments.

    View details for DOI 10.1101/gad.333997.119

    View details for PubMedID 31919192

    View details for PubMedCentralID PMC7000919

  • Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis. Journal of visualized experiments : JoVE Chen, J., McSwiggen, D., Ünal, E. 2018

    Abstract

    Single molecule fluorescence in situ hybridization (smFISH) is a powerful technique to study gene expression in single cells due to its ability to detect and count individual RNA molecules. Complementary to deep sequencing-based methods, smFISH provides information about the cell-to-cell variation in transcript abundance and the subcellular localization of a given RNA. Recently, we have used smFISH to study the expression of the gene NDC80 during meiosis in budding yeast, in which two transcript isoforms exist and the short transcript isoform has its entire sequence shared with the long isoform. To confidently identify each transcript isoform, we optimized known smFISH protocols and obtained high consistency and quality of smFISH data for the samples acquired during budding yeast meiosis. Here, we describe this optimized protocol, the criteria that we use to determine whether high quality of smFISH data is obtained, and some tips for implementing this protocol in other yeast strains and growth conditions.

    View details for DOI 10.3791/57774

    View details for PubMedID 29889208

    View details for PubMedCentralID PMC6101419

  • Transcription of a 5' extended mRNA isoform directs dynamic chromatin changes and interference of a downstream promoter. eLife Chia, M., Tresenrider, A., Chen, J., Spedale, G., Jorgensen, V., Ünal, E., van Werven, F. J. 2017; 6

    Abstract

    Cell differentiation programs require dynamic regulation of gene expression. During meiotic prophase in Saccharomyces cerevisiae, expression of the kinetochore complex subunit Ndc80 is downregulated by a 5' extended long undecoded NDC80 transcript isoform. Here we demonstrate a transcriptional interference mechanism that is responsible for inhibiting expression of the coding NDC80 mRNA isoform. Transcription from a distal NDC80 promoter directs Set1-dependent histone H3K4 dimethylation and Set2-dependent H3K36 trimethylation to establish a repressive chromatin state in the downstream canonical NDC80 promoter. As a consequence, NDC80 expression is repressed during meiotic prophase. The transcriptional mechanism described here is rapidly reversible, adaptable to fine-tune gene expression, and relies on Set2 and the Set3 histone deacetylase complex. Thus, expression of a 5' extended mRNA isoform causes transcriptional interference at the downstream promoter. We demonstrate that this is an effective mechanism to promote dynamic changes in gene expression during cell differentiation.

    View details for DOI 10.7554/eLife.27420

    View details for PubMedID 28906248

    View details for PubMedCentralID PMC5655139

  • Kinetochore inactivation by expression of a repressive mRNA. eLife Chen, J., Tresenrider, A., Chia, M., McSwiggen, D. T., Spedale, G., Jorgensen, V., Liao, H., van Werven, F. J., Ünal, E. 2017; 6

    Abstract

    Differentiation programs such as meiosis depend on extensive gene regulation to mediate cellular morphogenesis. Meiosis requires transient removal of the outer kinetochore, the complex that connects microtubules to chromosomes. How the meiotic gene expression program temporally restricts kinetochore function is unknown. We discovered that in budding yeast, kinetochore inactivation occurs by reducing the abundance of a limiting subunit, Ndc80. Furthermore, we uncovered an integrated mechanism that acts at the transcriptional and translational level to repress NDC80 expression. Central to this mechanism is the developmentally controlled transcription of an alternate NDC80 mRNA isoform, which itself cannot produce protein due to regulatory upstream ORFs in its extended 5' leader. Instead, transcription of this isoform represses the canonical NDC80 mRNA expression in cis, thereby inhibiting Ndc80 protein synthesis. This model of gene regulation raises the intriguing notion that transcription of an mRNA, despite carrying a canonical coding sequence, can directly cause gene repression.

    View details for DOI 10.7554/eLife.27417

    View details for PubMedID 28906249

    View details for PubMedCentralID PMC5655150