Jinwoo Shin

All Publications

• DNA Content and DNA Damage in Raw and Heat-Processed Foods. Journal of agricultural and food chemistry Shin, J., Jaruga, P., Dizdaroglu, M., Kool, E. T. 2025

  Abstract

  DNA in foods is a source of nucleotides that are salvaged by tissues as building blocks for chromosomal and mitochondrial DNA. A recent study provided preliminary evidence that high-temperature cooking damages the DNA in foods and suggested that certain forms of DNA damage can be taken up as nucleotides via metabolic salvage in cells and animals, directly incorporating genotoxic and mutagenic species into the host DNA. To assess potential risks, we surveyed DNA in 21 food ingredients, including plant- and meat-based foods in raw and roasted forms. We found a large variation in extractable DNA content, implying widely variable levels of consumption. Cooking resulted in greatly elevated levels of oxidative and deaminated DNA damage in nearly all foods, as indicated by 8-oxo-dG and dU nucleotides, with up to 250-fold increases. Studies of human cell lines found that incubation with these damaged nucleosides resulted in cytotoxicity and increased DNA double-strand break levels.

  View details for DOI 10.1021/acs.jafc.5c08138

  View details for PubMedID 41178059

• Wavelength engineerable porous organic polymer photosensitizers with protonation triggered ROS generation. Nature communications Shin, J., Kang, D. W., Lim, J. H., An, J. M., Kim, Y., Kim, J. H., Ji, M. S., Park, S., Kim, D., Lee, J. Y., Kim, J. S., Hong, C. S. 2023; 14 (1): 1498

  Abstract

  Engineering excitation wavelength of photosensitizers (PSs) for enhanced reactive oxygen species (ROS) generation has inspired new windows for opportunities, enabling investigation of previously impracticable biomedical and photocatalytic applications. However, controlling the wavelength corresponding to operating conditions remains challenging while maintaining high ROS generation. To address this challenge, we implement a wavelength-engineerable imidazolium-based porous organic photocatalytic ROS generation system (KUP system) via a cost-effective one-pot reaction. Remarkably, the optimal wavelength for maximum performance can be tuned by modifying the linker, generating ROS despite the absence of metal ions and covalently attached heavy atoms. We demonstrate that protonated polymerization exclusively enables photosensitization and closely interacts with oxygen related to the efficiency of photosensitizing. Furthermore, superior tumor eradication and biocompatibility of the KUP system were confirmed through bioassays. Overall, the results document an unprecedented polymerization method capable of engineering wavelength, providing a potential basis for designing nanoscale photosensitizers in various ROS-utilizing applications.

  View details for DOI 10.1038/s41467-023-37156-x

  View details for PubMedID 36932086