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  • Work
    julierod@stanford.edu
    University - Staff Department: Medicine - Med/Cardiovascular Medicine Position: Genetics Research Data Analyst

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  • Mail Code: 5406

All Publications

  • Characterisation of the biological response of Saccharomyces cerevisiae to the loss of an allele of the eukaryotic initiation factor 4A BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Venturi, V., Little, R., Bircham, P. W., Brito, J., Atkinson, P. H., Maass, D. R., Teesdale-Spittle, P. H. 2018; 496 (4): 1082-1087

    Abstract

    The translation initiation machinery is emerging as an important target for therapeutic intervention, with potential in the treatment of cancer, viral infections, and muscle wasting. Amongst the targets for pharmacological control of translation initiation is the eukaryotic initiation factor 4A (eIF4A), an RNA helicase that is essential for cap-dependent translation initiation. We set out to explore the system-wide impact of a reduction of functional eIF4A. To this end, we investigated the effect of deletion of TIF1, one of the duplicate genes that produce eIF4A in yeast, through synthetic genetic array interactions and system-wide changes in GFP-tagged protein abundances. We show that there is a biological response to deletion of the TIF1 gene that extends through the proteostasis network. Effects of the deletion are apparent in processes as distributed as chromatin remodelling, ribosome biogenesis, amino acid metabolism, and protein trafficking. The results from this study identify protein complexes and pathways that will make ideal targets for combination therapies with eIF4A inhibitors.

    View details for DOI 10.1016/j.bbrc.2018.01.137

    View details for Web of Science ID 000426336400011

    View details for PubMedID 29397069

  • Sex-related differences in age-associated downregulation of human ventricular myocardial beta(1)-adrenergic receptors JOURNAL OF HEART AND LUNG TRANSPLANTATION Lindenfeld, J., Cleveland, J. C., Kao, D. P., White, M., Wichman, S., Bristow, J. C., Peterson, V., Rodegheri-Brito, J., Korst, A., Blain-Nelson, P., Sederberg, J., Hunt, S. A., Gilbert, E. M., Ambardekar, A. V., Minobe, W., Port, J. D., Bristow, M. R. 2016; 35 (3): 352-361

    Abstract

    With increasing age, human ventricular myocardium exhibits selective downregulation of β1-adrenergic receptors (β1-ARs). We tested the hypothesis that sex differences exist in age-related changes in β1-ARs.Left (LV) and right (RV) ventricular tissue was obtained from 61 unplaceable potential organ donor hearts ages 1 to 71 years with no known cardiac history and from LVs removed from 56 transplant recipients with idiopathic dilated cardiomyopathy. β1-AR and β2-AR densities, the frequency of β1-AR389 gene variants, and β-AR function were determined.Sex had a marked effect on the age-related decrease in β1-ARs. Female LVs had more pronounced downregulation (by 42% [p < 0.001] vs 22% [p = 0.21] in 31 male LVs) comparing the youngest (average age, 15.3 ± 5.5 years) to the oldest (average age, 50.8 ± 9.1 years) sub-groups. On regression analyses, female LVs exhibited a closer relationship between β1-AR density and age (r = -0.78, p <0.001 vs r = -0.46, p = 0.009 in males), with a second-degree polynomial yielding the best fit. There was no statistically significant relationship of β1-ARs to age in female or male idiopathic dilated cardiomyopathy LVs.Sex affects age-related β-AR downregulation in normal human ventricles, with females exhibiting more profound decreases with increasing age. The curvilinear relationship between age and receptor density that plateaus around age 40 in women suggests an effect of sex hormones on β1-AR expression in the human heart.

    View details for DOI 10.1016/j.healun.2015.10.021

    View details for Web of Science ID 000372682900012