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  • Acute and inherited piRNA-mediated silencing in a rde-3 ribonucleotidyltransferase mutant. microPublication biology Priyadarshini, M., AlHarbi, S., Frokjar-Jensen, C. 2022; 2022


    We recently developed a piRNA-based silencing assay (piRNAi) to study small-RNA mediated epigenetic silencing: acute gene silencing is induced by synthetic piRNAs expressed from extra-chromosomal array and transgenerational inheritance can be quantified after array loss. The assay allows inheritance assays by injecting piRNAs directly into mutant animals and targeting endogenous genes ( e.g. , him-5 and him-8 ) with obvious phenotypes (increased male frequency). Here we demonstrate the piRNAi assay by quantifying acute and inherited silencing in the ribonucleotidyltransferase rde-3 (ne3370) mutant. In the absence of rde-3, acute silencing was reduced but still detectable, whereas inherited silencing was abolished.

    View details for DOI 10.17912/micropub.biology.000638

    View details for PubMedID 36188099