Stanford Advisors

Contact

  • Academic
    neals1@stanford.edu
    University - Scholar Department: Ophthalmology Position: Postdoctoral Scholar

All Publications

  • Mouse and Rat Oxygen-Induced Retinopathy Models to Study Vascular Features Seen in Retinopathy of Prematurity. Bio-protocol Tankersley, M. P., Beri, S., Ramshekar, A., Asare-Bediako, B., Shah, N. S., Karmoker, J. R., Huang, H. C., Hartnett, E. M. 2026; 16 (15): e5771

    Abstract

    Retinopathy of prematurity (ROP), a retinovascular disease, is a leading cause of childhood blindness worldwide. Given the constraints of studying molecular mechanisms in preterm infants, reproducible animal models are important to understand ROP pathophysiology. Mouse and rat oxygen-induced retinopathy (OIR) models are the most commonly used and recapitulate key vascular features seen in ROP. However, these models are susceptible to inherent variability that limits reproducibility, including inter-litter variability, consistency of oxygen delivery across experiments, retinal dissection technique, and immunohistochemistry. Here, we describe a comprehensive protocol for performing the most common mouse and rat OIR models, and procedures such as eye enucleation, retinal dissection and flat mounting, isolectin GS-IB4 staining, whole retina stitched fluorescence imaging from Z-stacks, and quantification of vascular features. This protocol provides important materials and procedural details to increase the reproducibility of the mouse and rat OIR models. Key features • Rat and mouse oxygen-induced retinopathy (OIR) models. • Eye enucleation of experimental rat and mouse pups. • Retinal flat mounting and immunostaining for rat and mouse eyes. • Image analysis of retinal flat mounts from rat and mouse eyes.

    View details for DOI 10.21769/BioProtoc.5771

    View details for PubMedID 42591369

    View details for PubMedCentralID PMC13461755

  • AI-Assisted Quantification of Retinal Vascular Parameters in Oxygen-Induced Retinopathy Models: A Net Approach Shah, N., Ramshekar, A., Asare-Bediako, B., Tankersley, M., Randolph, J. W. J., Whitney, J., Hartnett, M. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2026

    View details for Web of Science ID 001880673300040

  • Pathway Enrichment Analysis of 7-Ketocholesterol-Induced Endothelial-Mesenchymal Transiti in Choroidal Endothelial Cells: Insights into Age-Related Macular Degeneration Pathology Randolph, J. W. J., Ramshekar, A., Shah, N., Jacob, J., Tankersley, M., Beri, S., Karmoker, J., Hartnett, M. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2026

    View details for Web of Science ID 001880529000039

  • Descriptive Analysis of Retina Fellowship Program Directors in the United States in 2025 Parekh, B., Masoud, O., El-Omda, S., Shah, N. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2026

    View details for Web of Science ID 001880764500020

  • Retinal Neurovascular Development Requires Endothelial VEGFR2: Insights from the Mouse Oxygen induced Retinopathy Model Hartnett, M., Ramshekar, A., Asare-Bediako, B., Karmoker, J., Shah, N., Tankersley, M., Randolph, J. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2026

    View details for Web of Science ID 001879881100050

  • Systemic Immune Signaling Effects of Genipin: Insights From High-Throughput Whole-Blood CyTOF Huang, H., Anronikov, A., Randolph, J. W. J., Shah, N., Ramshekar, A., Asare-Bediako, B., Karmoker, J., Stevenson, D. K., Gaudilliere, B., Hartnett, M. ASSOC RESEARCH VISION OPHTHALMOLOGY INC. 2026

    View details for Web of Science ID 001880706600046