Patrick Almhjell

All Publications

• The beta-subunit of tryptophan synthase is a latent tyrosine synthase. Nature chemical biology Almhjell, P. J., Johnston, K. E., Porter, N. J., Kennemur, J. L., Bhethanabotla, V. C., Ducharme, J., Arnold, F. H. 2024

  Abstract

  Aromatic amino acids and their derivatives are diverse primary and secondary metabolites with critical roles in protein synthesis, cell structure and integrity, defense and signaling. All de novo aromatic amino acid production relies on a set of ancient and highly conserved chemistries. Here we introduce a new enzymatic transformation for L-tyrosine synthesis by demonstrating that the beta-subunit of tryptophan synthase-which natively couples indole and L-serine to form L-tryptophan-can act as a latent 'tyrosine synthase'. A single substitution of a near-universally conserved catalytic residue unlocks activity toward simple phenol analogs and yields exclusive para carbon-carbon bond formation to furnish L-tyrosines. Structural and mechanistic studies show how a new active-site water molecule orients phenols for a nonnative mechanism of alkylation, with additional directed evolution resulting in a net >30,000-fold rate enhancement. This new biocatalyst can be used to efficiently prepare valuable L-tyrosine analogs at gram scales and provides the missing chemistry for a conceptually different pathway to L-tyrosine.

  View details for DOI 10.1038/s41589-024-01619-z

  View details for PubMedID 38744987