Education & Certifications


  • M.S., Stanford University, Stanford, CA, Bioengineering
  • B.Eng. (Hons.), Nanyang Technological University, Singapore, Chemical and Biomolecular Engineering

Stanford Advisors


All Publications


  • Robotic Pill for Biomarker and Fluid Sampling in the Gastrointestinal Tract ADVANCED INTELLIGENT SYSTEMS Soto, F., Purcell, E., Ozen, M., Sinawang, P., Wang, J., Akin, D., Demirci, U. 2022
  • Progress and challenges in biomarker enrichment for cancer early detection Progress in Biomedical Engineering Sinawang, P., Soto, F., Ozen, M. O., Akin, D., Demirci, U. 2021; 3 (4)

    View details for DOI 10.1088/2516-1091/ac1ea3

  • Cost-Effectiveness Analysis of Fenestrated Endovascular Aneurysm Repair Compared With Open Surgical Repair for Patients With Juxtarenal Abdominal Aortic Aneurysms George, E. L., Nardacci, L., Sinawang, P., Rao, I., Owens, D. K., Garcia-Toca, M. MOSBY-ELSEVIER. 2019: E244–E245
  • TEMPO-based immuno-lateral flow quantitative detection of dengue NS1 protein SENSORS AND ACTUATORS B-CHEMICAL Sinawang, P., Fajs, L., Elouarzaki, K., Nugraha, J., Marks, R. S. 2018; 259: 354–63
  • Photoinducible silane diazirine as an effective crosslinker in the construction of a chemiluminescent immunosensor targeting a model E. coli analyte SENSORS AND ACTUATORS B-CHEMICAL Ye, K., Sinawang, P., Tok, A., Marks, R. S. 2018; 256: 234–42
  • Rapid and label-free electrochemical DNA biosensor for detecting hepatitis A virus BIOSENSORS & BIOELECTRONICS Manzano, M., Viezzi, S., Mazerat, S., Marks, R. S., Vidic, J. 2018; 100: 89–95

    Abstract

    Diagnostic systems that can deliver highly specific and sensitive detection of hepatitis A virus (HAV) in food and water are of particular interest in many fields including food safety, biosecurity and control of outbreaks. Our aim was the development of an electrochemical method based on DNA hybridization to detect HAV. A ssDNA probe specific for HAV (capture probe) was designed and tested on DNAs from various viral and bacterial samples using Nested-Reverse Transcription Polymerase Chain Reaction (nRT-PCR). To develop the electrochemical device, a disposable gold electrode was functionalized with the specific capture probe and tested on complementary ssDNA and on HAV cDNA. The DNA hybridization on the electrode was measured through the monitoring of the oxidative peak potential of the indicator tripropylamine by cyclic voltammetry. To prevent non-specific binding the gold surface was treated with 3% BSA before detection. High resolution atomic force microscopy (AFM) confirmed the efficiency of electrode functionalization and on-electrode hybridization. The proposed device showed a limit of detection of 0.65pM for the complementary ssDNA and 6.94fg/µL for viral cDNA. For a comparison, nRT-PCR quantified the target HAV cDNA with a limit of detection of 6.4fg/µL. The DNA-sensor developed can be adapted to a portable format to be adopted as an easy-to- use and low cost method for screening HAV in contaminated food and water. In addition, it can be useful for rapid control of HAV infections as it takes only a few minutes to provide the results.

    View details for DOI 10.1016/j.bios.2017.08.043

    View details for Web of Science ID 000416187600013

    View details for PubMedID 28865923

  • Electrochemical impedimetric detection of stroke biomarker NT-proBNP using disposable screen-printed gold electrodes EUROBIOTECH JOURNAL Sinawang, P., Harpaz, D., Fajs, L., Seet, R., Tok, A., Marks, R. S. 2017; 1 (2): 165–76
  • Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein BIOSENSORS & BIOELECTRONICS Sinawang, P., Rai, V., Ionescu, R. E., Marks, R. S. 2016; 77: 400–408

    Abstract

    An Electrochemical Lateral Flow Immunosensor (ELFI) is developed combining screen-printed gold electrodes (SPGE) enabling quantification together with the convenience of a lateral flow test strip. A cellulose glassy fiber paper conjugate pad retains the marker immunoelectroactive nanobeads which will bind to the target analyte of interest. The specific immunorecognition event continues to occur along the lateral flow bed until reaching the SPGE-capture antibodies at the end of the cellulosic lateral flow strip. The rationale of the immunoassay consists in the analyte antigen NS1 protein being captured selectively and specifically by the dengue NS1 antibody conjugated onto the immunonanobeads thus forming an immunocomplex. With the aid of a running buffer, the immunocomplexes flow and reach the immuno-conjugated electrode surface and form specific sandwich-type detection due to specific, molecular recognition, while unbound beads move along past the electrodes. The successful sandwich immunocomplex formation is then recorded electrochemically. Specific detection of NS1 is translated into an electrochemical signal contributed by a redox label present on the bead-immobilized detection dengue NS1 antibody while a proportional increase of faradic current is observed with increase in analyte NS1 protein concentration. The first generation ELFI prototype is simply assembled in a cassette and successfully demonstrates wide linear range over a concentration range of 1-25 ng/mL with an ultrasensitive detection limit of 0.5 ng/mL for the qualitative and quantitative detection of analyte dengue NS1 protein.

    View details for DOI 10.1016/j.bios.2015.09.048

    View details for Web of Science ID 000366766900058

    View details for PubMedID 26433352

  • Lateral Flow Immunoassays - from Paper Strip to Smartphone Technology ELECTROANALYSIS Eltzov, E., Guttel, S., Kei, A., Sinawang, P., Ionescu, R. E., Marks, R. S. 2015; 27 (9): 2116–30
  • Optimizing aerosolization efficiency of dry-powder aggregates of thermally-sensitive polymeric nanoparticles produced by spray-freeze-drying POWDER TECHNOLOGY Kho, K., Hadinoto, K. 2011; 214 (1): 169–76