Stefano Cestellos Blanco

All Publications

• Report ATP production from electricity with a new-to-nature electrobiological module JOULE Luo, S., Adam, D., Giaveri, S., Barthel, S., Cestellos-Blanco, S., Hege, D., Paczia, N., Castaneda-Losada, L., Klose, M., Arndt, F., Heider, J., Erb, T. J. 2023; 7 (8): 1745-1758

  View details for DOI 10.1016/j.joule.2023.07.012

  View details for Web of Science ID 001097787900001

• Towards a Biomanufactory on Mars FRONTIERS IN ASTRONOMY AND SPACE SCIENCES Berliner, A. J., Hilzinger, J. M., Abel, A. J., McNulty, M. J., Makrygiorgos, G., Averesch, N. J. H., Sen Gupta, S., Benvenuti, A., Caddell, D. F., Cestellos-Blanco, S., Doloman, A., Friedline, S., Ho, D., Gu, W., Hill, A., Kusuma, P., Lipsky, I., Mirkovic, M., Meraz, J., Pane, V., Sander, K. B., Shi, F., Skerker, J. M., Styer, A., Valgardson, K., Wetmore, K., Woo, S., Xiong, Y., Yates, K., Zhang, C., Zhen, S., Bugbee, B., Clark, D. S., Coleman-Derr, D., Mesbah, A., Nandi, S., Waymouth, R. M., Yang, P., Criddle, C. S., McDonald, K. A., Seefeldt, L. C., Menezes, A. A., Arkin, A. P. 2021; 8

  View details for DOI 10.3389/fspas.2021.711550

  View details for Web of Science ID 000680691100001

• Nontoxic nanopore electroporation for effective intracellular delivery of biological macromolecules. Proceedings of the National Academy of Sciences of the United States of America Cao, Y., Ma, E., Cestellos-Blanco, S., Zhang, B., Qiu, R., Su, Y., Doudna, J. A., Yang, P. 2019; 116 (16): 7899–7904

  Abstract

  We present a simple nanopore-electroporation (NanoEP) platform for delivery of nucleic acids, functional protein, and Cas9 single-guide RNA ribonucleoproteins into both adherent and suspension cells with up to 80% delivery efficiency and >95% cell viability. Low-voltage electric pulses permeabilize a small area of cell membrane as a cell comes into close contact with the nanopores. The biomolecule cargo is then electrophoretically drawn into the cells through the nanopores. In addition to high-performance delivery with low cell toxicity, the NanoEP system does not require specialized buffers, expensive materials, complicated fabrication processes, or cell manipulation; it simply consists of a generic nanopore-embedded water-filter membrane and a low-voltage square-wave generator. Ultimately, the NanoEP platform offers an effective and flexible method for universal intracellular delivery.

  View details for PubMedID 30923112

• Roadmap on semiconductor-cell biointerfaces. Physical biology Tian, B., Xu, S., Rogers, J. A., Cestellos-Blanco, S., Yang, P., Carvalho-de-Souza, J. L., Bezanilla, F., Liu, J., Bao, Z., Hjort, M., Cao, Y., Melosh, N., Lanzani, G., Benfenati, F., Galli, G., Gygi, F., Kautz, R., Gorodetsky, A. A., Kim, S. S., Lu, T. K., Anikeeva, P., Cifra, M., Krivosudsky, O., Havelka, D., Jiang, Y. 2018; 15 (3): 031002

  Abstract

  This roadmap outlines the role semiconductor-based materials play in understanding the complex biophysical dynamics at multiple length scales, as well as the design and implementation of next-generation electronic, optoelectronic, and mechanical devices for biointerfaces. The roadmap emphasizes the advantages of semiconductor building blocks in interfacing, monitoring, and manipulating the activity of biological components, and discusses the possibility of using active semiconductor-cell interfaces for discovering new signaling processes in the biological world.

  View details for DOI 10.1088/1478-3975/aa9f34

  View details for PubMedID 29205173

• Miniaturized Antimicrobial Susceptibility Test by Combining Concentration Gradient Generation and Rapid Cell Culturing. Antibiotics (Basel, Switzerland) Kim, S. C., Cestellos-Blanco, S., Inoue, K., Zare, R. N. 2015; 4 (4): 455-466

  Abstract

  Effective treatment of bacterial infection relies on timely diagnosis and proper prescription of antibiotic drugs. The antimicrobial susceptibility test (AST) is one of the most crucial experimental procedures, providing the baseline information for choosing effective antibiotic agents and their dosages. Conventional methods, however, require long incubation times or significant instrumentation costs to obtain test results. We propose a lab-on-a-chip approach to perform AST in a simple, economic, and rapid manner. Our assay platform miniaturizes the standard broth microdilution method on a microfluidic device (20 × 20 mm) that generates an antibiotic concentration gradient and delivers antibiotic-containing culture media to eight 30-nL chambers for cell culture. When tested with 20 μL samples of a model bacterial strain (E. coli ATCC 25922) treated with ampicillin or streptomycin, our method allows for the determination of minimum inhibitory concentrations consistent with the microdilution test in three hours, which is almost a factor of ten more rapid than the standard method.

  View details for DOI 10.3390/antibiotics4040455

  View details for PubMedID 27025635

  View details for PubMedCentralID PMC4790307