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All Publications


  • Somatic activating mutation in Phosphoinositide 3-kinase in a plexiform lesion of a patient with hereditary hemorrhagic telangiectasia and pulmonary arterial hypertension Schimmel, K., DeBose-Scarlett, E., Qi, Y., Tan, S., Mastrodicasa, D., Peruzzi, N., Tran-Lundmark, K., Wu, J., Marchuk, D., Spiekerkoetter, E. LIPPINCOTT WILLIAMS & WILKINS. 2025
  • Protocol for efficient generation of human artery and vein endothelial cells from pluripotent stem cells. STAR protocols Loh, K. M., Zheng, S. L., Liu, K. J., Yin, Q., Amir-Ugokwe, Z. A., Jha, S. K., Qi, Y., Wazny, V. K., Nguyen, A. T., Chen, A., Njunkeng, F. M., Cheung, C., Spiekerkoetter, E., Red-Horse, K., Ang, L. T. 2024; 6 (1): 103494

    Abstract

    Blood vessels permeate all organs and execute myriad roles in health and disease. Here, we present a protocol to efficiently generate human artery and vein endothelial cells (ECs) from pluripotent stem cells within 3-4 days of differentiation. We delineate how to seed human pluripotent stem cells and sequentially differentiate them into primitive streak, lateral mesoderm, and either artery or vein ECs. We differentiate stem cells in defined, serum-free culture media in monolayers, without feeder cells or genetic manipulations. For complete details on the use and execution of this protocol, please refer to Ang et al. 1.

    View details for DOI 10.1016/j.xpro.2024.103494

    View details for PubMedID 39705146