Shawn Costello

All Publications

• An automated liquid jet for fluorescence dosimetry and microsecond radiolytic labeling of proteins. Communications biology Rosi, M., Russell, B., Kristensen, L. G., Farquhar, E. R., Jain, R., Abel, D., Sullivan, M., Costello, S. M., Dominguez-Martin, M. A., Chen, Y., Marqusee, S., Petzold, C. J., Kerfeld, C. A., DePonte, D. P., Farahmand, F., Gupta, S., Ralston, C. Y. 2022; 5 (1): 866

  Abstract

  X-ray radiolytic labeling uses broadband X-rays for in situ hydroxyl radical labeling to map protein interactions and conformation. High flux density beams are essential to overcome radical scavengers. However, conventional sample delivery environments, such as capillary flow, limit the use of a fully unattenuated focused broadband beam. An alternative is to use a liquid jet, and we have previously demonstrated that use of this form of sample delivery can increase labeling by tenfold at an unfocused X-ray source. Here we report the first use of a liquid jet for automated inline quantitative fluorescence dosage characterization and sample exposure at a high flux density microfocused synchrotron beamline. Our approach enables exposure times in single-digit microseconds while retaining a high level of side-chain labeling. This development significantly boosts the method's overall effectiveness and efficiency, generates high-quality data, and opens up the arena for high throughput and ultrafast time-resolved in situ hydroxyl radical labeling.

  View details for DOI 10.1038/s42003-022-03775-1

  View details for PubMedID 36008591

• Mapping Binding Interfaces and Allosteric Changes in the SARS-Cov-2 Spike Protein using Hydrogen/Deuterium Exchange Mass Spectrometry Costello, S. M., Hobbs, H. T., Shoemaker, S. R., Powell, A. E., Lim, S. A., Wells, J. A., Kim, P. S., Pak, J. E., Marqusee, S. CELL PRESS. 2021: 127A–128A

  View details for Web of Science ID 000629601400615