Polly Fordyce is an Assistant Professor in the Genetics Department at Stanford, as well as a Faculty Fellow in ChEM-H. Her lab will focus on using microfluidic tools to make quantitative measurements of transcription factor specificity and developing new tools to allow the creation of large peptide and combinatorial chemistry libraries. Polly earned her PhD in the Physics Department at Stanford for single-molecule studies of kinesin family proteins in Steve Block's laboratory. As a postdoctoral fellow, she worked in Joe DeRisi's laboratory at UCSF developing microfluidic tools for characterizing transcription factor binding and using them to characterize proteins from both yeast and humans. She is starting her own lab at Stanford in September 2014.
Honors & Awards
Pathway to Independence Award (K99), NIH (2012-2014)
Helen Hay Whitney Postdoctoral Fellowship, Helen Hay Whitney Foundation (2008-2011)
G. J. Lieberman Fellow, Stanford University (2003-2004)
Graduate Research Fellow, National Science Foundation (2002-2005)
Postdoctoral Fellow, University of California San Francisco, Biophysics (2014)
Ph.D., Stanford University, Physics (2007)
B.A., University of Colorado at Boulder, Physics, Biology (2000)
Current Research and Scholarly Interests
The Fordyce Lab is focused on developing new instrumentation and assays for making quantitative, systems-scale biophysical measurements of molecular interactions. Current research in the lab is focused on two main areas: using microfluidic tools we have developed to build ground-up quantitative models of how gene expression is regulated, and developing new tools to transform how scientists explore protein-protein interactions.
Microfluidic studies of transcription factor specificity:
Cutting-edge technologies have identified genomic DNA regulatory elements and revealed the binding preferences for many transcription factors. However, our ability to predict in vivo patterns of transcription factor binding from DNA sequence alone remains poor, and measured in vivo binding often cannot explain gene expression patterns, even for well-studied eukaryotic promoters. Improving our understanding of these processes could have far-reaching impacts for revealing how mutations in regulatory elements cause disease and for designing transcriptional circuits for use in synthetic biology. Using a microfluidic technique we have recently developed for making high-throughput, quantitative measurements of transcription factor binding interactions (MITOMI 2.0), we propose to use a "ground-up" approach to reverse engineer transcriptional regulation by systematically adding in components and observing how they influence steady-state occupancies and binding kinetics.
Spectral encoding for biological multiplexing:
Biological multiplexing allows using very small amounts of samples to test for many different things in parallel. Bead-based multiplexing has many advantages, but poses a central challenge: beads must be encoded in some way. We have recently developed new microfluidic methods to produce beads that are spectrally encoded via the ratiometric incorporation of different lanthanide nanonanophosphors. These materials have unique spectral signatures, meaning that beads can later be imaged to "read" the embedded codes. We have previously demonstrated the ability to make up to 82 distinct codes using three lanthanide species (Europium, Samarium, and Dysprosium). We are currently working on expanding our code space to include up to 1,000 distinct spectral codes by using additional lanthanide species, as well as on functionalizing beads for downstream assays.
- Microfluidic Device Laboratory
BIOE 301D, GENE 207 (Win)
Independent Studies (11)
- Bioengineering Problems and Experimental Investigation
BIOE 191 (Sum)
- Directed Investigation
BIOE 392 (Aut, Win, Spr, Sum)
- Directed Reading in Biophysics
BIOPHYS 399 (Win, Spr)
- Directed Reading in Genetics
GENE 299 (Aut, Win, Spr)
- Directed Study
BIOE 391 (Aut)
- Graduate Research
BIOPHYS 300 (Win, Spr)
- Graduate Research
GENE 399 (Aut, Win, Spr, Sum)
- Medical Scholars Research
GENE 370 (Aut, Win, Spr)
- Out-of-Department Advanced Research Laboratory in Experimental Biology
BIO 199X (Aut, Win, Spr)
- Supervised Study
GENE 260 (Aut, Win, Spr)
- Undergraduate Research
GENE 199 (Aut, Win, Spr)
- Bioengineering Problems and Experimental Investigation
- Prior Year Courses
Graduate and Fellowship Programs
How duplicated transcription regulators can diversify to govern the expression of nonoverlapping sets of genes
GENES & DEVELOPMENT
2014; 28 (12): 1272-1277
The duplication of transcription regulators can elicit major regulatory network rearrangements over evolutionary timescales. However, few examples of duplications resulting in gene network expansions are understood in molecular detail. Here we show that four Candida albicans transcription regulators that arose by successive duplications have differentiated from one another by acquiring different intrinsic DNA-binding specificities, different preferences for half-site spacing, and different associations with cofactors. The combination of these three mechanisms resulted in each of the four regulators controlling a distinct set of target genes, which likely contributed to the adaption of this fungus to its human host. Our results illustrate how successive duplications and diversification of an ancestral transcription regulator can underlie major changes in an organism's regulatory circuitry.
View details for DOI 10.1101/gad.242271.114
View details for Web of Science ID 000337991000002
View details for PubMedID 24874988
Structure of the transcriptional network controlling white-opaque switching in Candida albicans
2013; 90 (1): 22-35
The human fungal pathogen Candida albicans can switch between two phenotypic cell types, termed 'white' and 'opaque'. Both cell types are heritable for many generations, and the switch between the two types occurs epigenetically, that is, without a change in the primary DNA sequence of the genome. Previous work identified six key transcriptional regulators important for white-opaque switching: Wor1, Wor2, Wor3, Czf1, Efg1, and Ahr1. In this work, we describe the structure of the transcriptional network that specifies the white and opaque cell types and governs the ability to switch between them. In particular, we use a combination of genome-wide chromatin immunoprecipitation, gene expression profiling, and microfluidics-based DNA binding experiments to determine the direct and indirect regulatory interactions that form the switch network. The six regulators are arranged together in a complex, interlocking network with many seemingly redundant and overlapping connections. We propose that the structure (or topology) of this network is responsible for the epigenetic maintenance of the white and opaque states, the switching between them, and the specialized properties of each state.
View details for DOI 10.1111/mmi.12329
View details for Web of Science ID 000324950600003
View details for PubMedID 23855748
Microfluidic affinity and ChIP-seq analyses converge on a conserved FOXP2-binding motif in chimp and human, which enables the detection of evolutionarily novel targets
NUCLEIC ACIDS RESEARCH
2013; 41 (12): 5991-6004
The transcription factor forkhead box P2 (FOXP2) is believed to be important in the evolution of human speech. A mutation in its DNA-binding domain causes severe speech impairment. Humans have acquired two coding changes relative to the conserved mammalian sequence. Despite intense interest in FOXP2, it has remained an open question whether the human protein's DNA-binding specificity and chromatin localization are conserved. Previous in vitro and ChIP-chip studies have provided conflicting consensus sequences for the FOXP2-binding site. Using MITOMI 2.0 microfluidic affinity assays, we describe the binding site of FOXP2 and its affinity profile in base-specific detail for all substitutions of the strongest binding site. We find that human and chimp FOXP2 have similar binding sites that are distinct from previously suggested consensus binding sites. Additionally, through analysis of FOXP2 ChIP-seq data from cultured neurons, we find strong overrepresentation of a motif that matches our in vitro results and identifies a set of genes with FOXP2 binding sites. The FOXP2-binding sites tend to be conserved, yet we identified 38 instances of evolutionarily novel sites in humans. Combined, these data present a comprehensive portrait of FOXP2's-binding properties and imply that although its sequence specificity has been conserved, some of its genomic binding sites are newly evolved.
View details for DOI 10.1093/nar/gkt259
View details for Web of Science ID 000321057100012
View details for PubMedID 23625967
Identification and characterization of a previously undescribed family of sequence-specific DNA-binding domains
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2013; 110 (19): 7660-7665
Sequence-specific DNA-binding proteins are among the most important classes of gene regulatory proteins, controlling changes in transcription that underlie many aspects of biology. In this work, we identify a transcriptional regulator from the human fungal pathogen Candida albicans that binds DNA specifically but has no detectable homology with any previously described DNA- or RNA-binding protein. This protein, named White-Opaque Regulator 3 (Wor3), regulates white-opaque switching, the ability of C. albicans to switch between two heritable cell types. We demonstrate that ectopic overexpression of WOR3 results in mass conversion of white cells to opaque cells and that deletion of WOR3 affects the stability of opaque cells at physiological temperatures. Genome-wide chromatin immunoprecipitation of Wor3 and gene expression profiling of a wor3 deletion mutant strain indicate that Wor3 is highly integrated into the previously described circuit regulating white-opaque switching and that it controls a subset of the opaque transcriptional program. We show by biochemical, genetic, and microfluidic experiments that Wor3 binds directly to DNA in a sequence-specific manner, and we identify the set of cis-regulatory sequences recognized by Wor3. Bioinformatic analyses indicate that the Wor3 family arose more recently in evolutionary time than most previously described DNA-binding domains; it is restricted to a small number of fungi that include the major fungal pathogens of humans. These observations show that new families of sequence-specific DNA-binding proteins may be restricted to small clades and suggest that current annotations--which rely on deep conservation--underestimate the fraction of genes coding for transcriptional regulators.
View details for DOI 10.1073/pnas.1221734110
View details for Web of Science ID 000319327700041
View details for PubMedID 23610392
Programmable microfluidic synthesis of spectrally encoded microspheres
LAB ON A CHIP
2012; 12 (22): 4716-4723
Spectrally encoded fluorescent beads are an attractive platform for assay miniaturization and multiplexing in the biological sciences. Here, we synthesize hydrophilic PEG-acrylate polymer beads encoded with lanthanide nanophosphors using a fully automated microfluidic synthesis device. These beads are encoded by including varying amounts of two lanthanide nanophosphors relative to a third reference nanophosphor to generate 24 distinct ratios. These codes differ by less than 3% from their target values and can be distinguished from each other with an error rate of <0.1%. The encoded bead synthesis strategy we have used is readily extensible to larger numbers of codes, potentially up to millions, providing a new platform technology for assay multiplexing.
View details for DOI 10.1039/c2lc40699c
View details for Web of Science ID 000310865200017
View details for PubMedID 23042484
Basic leucine zipper transcription factor Hac1 binds DNA in two distinct modes as revealed by microfluidic analyses
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2012; 109 (45): E3084-E3093
A quantitative understanding of how transcription factors interact with genomic target sites is crucial for reconstructing transcriptional networks in vivo. Here, we use Hac1, a well-characterized basic leucine zipper (bZIP) transcription factor involved in the unfolded protein response (UPR) as a model to investigate interactions between bZIP transcription factors and their target sites. During the UPR, the accumulation of unfolded proteins leads to unconventional splicing and subsequent translation of HAC1 mRNA, followed by transcription of UPR target genes. Initial candidate-based approaches identified a canonical cis-acting unfolded protein response element (UPRE-1) within target gene promoters; however, subsequent studies identified a large set of Hac1 target genes lacking this UPRE-1 and containing a different motif (UPRE-2). Using a combination of unbiased and directed microfluidic DNA binding assays, we established that Hac1 binds in two distinct modes: (i) to short (6-7 bp) UPRE-2-like motifs and (ii) to significantly longer (11-13 bp) extended UPRE-1-like motifs. Using a genetic screen, we demonstrate that a region of extended homology N-terminal to the basic DNA binding domain is required for this dual site recognition. These results establish Hac1 as the first bZIP transcription factor known to adopt more than one binding mode and unify previously conflicting and discrepant observations of Hac1 function into a cohesive model of UPR target gene activation. Our results also suggest that even structurally simple transcription factors can recognize multiple divergent target sites of very different lengths, potentially enriching their downstream target repertoire.
View details for DOI 10.1073/pnas.1212457109
View details for Web of Science ID 000311156700009
View details for PubMedID 23054834
Systematic characterization of feature dimensions and closing pressures for microfluidic valves produced via photoresist reflow
LAB ON A CHIP
2012; 12 (21): 4287-4295
Multilayer soft lithography (MSL) provides a convenient and low-cost method for fabricating poly(dimethyl siloxane) (PDMS) microfluidic devices with on-chip valves for automated and precise control of fluid flow. MSL casting molds for flow channels typically incorporate small patches of rounded positive photoresist at valve locations to achieve the rounded cross-sectional profile required for these valves to function properly. Despite the importance of these rounded features for device performance, a comprehensive characterization of how the rounding process affects feature dimensions and closing pressures has been lacking. Here, we measure valve dimensions both before and after rounding and closing pressures for 120 different valve widths and lengths at post-rounding heights between 15 and 84 μm, for a total of 1200 different geometries spanning a wide range of useful sizes. We find that valve height and width after rounding depend strongly on valve aspect ratios, with these effects becoming more pronounced for taller and narrower features. Based on the measured data, we provide a simple fitted model and an online tool for estimating the pre-rounding dimensions needed to achieve desired post-rounding dimensions. We also find that valve closing pressures are well explained by modelling valve membranes in a manner analogous to a suspension bridge, shedding new light on device physics and providing a practical model for estimating closing pressures during device design.
View details for DOI 10.1039/c2lc40414a
View details for Web of Science ID 000310916100012
View details for PubMedID 22930180
De novo identification and biophysical characterization of transcription-factor binding sites with microfluidic affinity analysis
2010; 28 (9): 970-976
Gene expression is regulated in part by protein transcription factors that bind target regulatory DNA sequences. Predicting DNA binding sites and affinities from transcription factor sequence or structure is difficult; therefore, experimental data are required to link transcription factors to target sequences. We present a microfluidics-based approach for de novo discovery and quantitative biophysical characterization of DNA target sequences. We validated our technique by measuring sequence preferences for 28 Saccharomyces cerevisiae transcription factors with a variety of DNA-binding domains, including several that have proven difficult to study by other techniques. For each transcription factor, we measured relative binding affinities to oligonucleotides covering all possible 8-bp DNA sequences to create a comprehensive map of sequence preferences; for four transcription factors, we also determined absolute affinities. We expect that these data and future use of this technique will provide information essential for understanding transcription factor specificity, improving identification of regulatory sites and reconstructing regulatory interactions.
View details for DOI 10.1038/nbt.1675
View details for Web of Science ID 000281719100024
View details for PubMedID 20802496
View details for PubMedCentralID PMC2937095
Individual dimers of the mitotic kinesin motor Eg5 step processively and support substantial loads in vitro
NATURE CELL BIOLOGY
2006; 8 (5): 470-U89
Eg5, a member of the kinesin superfamily of microtubule-based motors, is essential for bipolar spindle assembly and maintenance during mitosis, yet little is known about the mechanisms by which it accomplishes these tasks. Here, we used an automated optical trapping apparatus in conjunction with a novel motility assay that employed chemically modified surfaces to probe the mechanochemistry of Eg5. Individual dimers, formed by a recombinant human construct Eg5-513-5His, stepped processively along microtubules in 8-nm increments, with short run lengths averaging approximately eight steps. By varying the applied load (with a force clamp) and the ATP concentration, we found that the velocity of Eg5 was slower and less sensitive to external load than that of conventional kinesin, possibly reflecting the distinct demands of spindle assembly as compared with vesicle transport. The Eg5-513-5His velocity data were described by a minimal, three-state model where a force-dependent transition follows nucleotide binding.
View details for DOI 10.1038/ncb1394
View details for Web of Science ID 000237299400010
View details for PubMedID 16604065
Eg5 steps it up!
Understanding how molecular motors generate force and move microtubules in mitosis is essential to understanding the physical mechanism of cell division. Recent measurements have shown that one mitotic kinesin superfamily member, Eg5, is mechanically processive and capable of crosslinking and sliding microtubules in vitro. In this review, we highlight recent work that explores how Eg5 functions under load, with an emphasis on the nanomechanical properties of single enzymes.
View details for DOI 10.1186/1747-1028-1-31
View details for Web of Science ID 000207723600031
View details for PubMedID 17173688
Simultaneous, coincident optical trapping and single-molecule fluorescence
2004; 1 (2): 133-139
We constructed a microscope-based instrument capable of simultaneous, spatially coincident optical trapping and single-molecule fluorescence. The capabilities of this apparatus were demonstrated by studying the force-induced strand separation of a dye-labeled, 15-base-pair region of double-stranded DNA (dsDNA), with force applied either parallel ('unzipping' mode) or perpendicular ('shearing' mode) to the long axis of the region. Mechanical transitions corresponding to DNA hybrid rupture occurred simultaneously with discontinuous changes in the fluorescence emission. The rupture force was strongly dependent on the direction of applied force, indicating the existence of distinct unbinding pathways for the two force-loading modes. From the rupture force histograms, we determined the distance to the thermodynamic transition state and the thermal off rates in the absence of load for both processes.
View details for DOI 10.1038/NMETH714
View details for Web of Science ID 000226753800017
View details for PubMedID 15782176
Stepping and stretching - How kinesin uses internal strain to walk processively
JOURNAL OF BIOLOGICAL CHEMISTRY
2003; 278 (20): 18550-18556
The ability of kinesin to travel long distances on its microtubule track without dissociating has led to a variety of models to explain how this remarkable degree of processivity is maintained. All of these require that the two motor domains remain enzymatically "out of phase," a behavior that would ensure that, at any given time, one motor is strongly attached to the microtubule. The maintenance of this coordination over many mechanochemical cycles has never been explained, because key steps in the cycle could not be directly observed. We have addressed this issue by applying several novel spectroscopic approaches to monitor motor dissociation, phosphate release, and nucleotide binding during processive movement by a dimeric kinesin construct. Our data argue that the major effect of the internal strain generated when both motor domains of kinesin bind the microtubule is to block ATP from binding to the leading motor. This effect guarantees the two motor domains remain out of phase for many mechanochemical cycles and provides an efficient and adaptable mechanism for the maintenance of processive movement.
View details for DOI 10.1074/jbc.M300849200
View details for Web of Science ID 000182838300126
View details for PubMedID 12626516
Combined optical trapping and single-molecule fluorescence.
Journal of biology
2003; 2 (1): 6-?
Two of the mainstay techniques in single-molecule research are optical trapping and single-molecule fluorescence. Previous attempts to combine these techniques in a single experiment - and on a single macromolecule of interest - have met with little success, because the light intensity within an optical trap is more than ten orders of magnitude greater than the light emitted by a single fluorophore. Instead, the two techniques have been employed sequentially, or spatially separated by distances of several micrometers within the sample, imposing experimental restrictions that limit the utility of the combined method. Here, we report the development of an instrument capable of true, simultaneous, spatially coincident optical trapping and single-molecule fluorescence.We demonstrate the capability of the apparatus by studying force-induced strand separation of a rhodamine-labeled, 15 base-pair segment of double-stranded DNA, with force applied perpendicular to the axis of the DNA molecule. As expected, we observed abrupt mechanical transitions corresponding to the unzipping of DNA at a critical force. Transitions occurred concomitant with changes in the fluorescence of dyes attached at the duplex ends, which became unquenched upon strand separation.Through careful optical design, the use of high-performance spectral notch filters, a judicious choice of fluorophores, and the rapid acquisition of data gained by computer-automating the experiment, it is possible to perform combined optical trapping and single-molecule fluorescence. This opens the door to many types of experiment that employ optical traps to supply controlled external loads while fluorescent molecules report concurrent information about macromolecular structure.
View details for PubMedID 12733997