Doctor of Philosophy, Tsinghua University (2020)
Bachelor of Science, Sichuan University (2013)
PhD, Tsinghua University, Microbiology, Molecular Biology (2020)
BS, Sichuan University, Biology (2013)
Maria Barna, Postdoctoral Faculty Sponsor
RAPIDASH: A tag-free enrichment of ribosome-associated proteins reveals compositional dynamics in embryonic tissues and stimulated macrophages.
bioRxiv : the preprint server for biology
Ribosomes are emerging as direct regulators of gene expression, with ribosome-associated proteins (RAPs) allowing ribosomes to modulate translational control. However, a lack of technologies to enrich RAPs across many sample types has prevented systematic analysis of RAP number, dynamics, and functions. Here, we have developed a label-free methodology called RAPIDASH to enrich ribosomes and RAPs from any sample. We applied RAPIDASH to mouse embryonic tissues and identified hundreds of potential RAPs, including DHX30 and LLPH, two forebrain RAPs important for neurodevelopment. We identified a critical role of LLPH in neural development that is linked to the translation of genes with long coding sequences. Finally, we characterized ribosome composition remodeling during immune activation and observed extensive changes post-stimulation. RAPIDASH has therefore enabled the discovery of RAPs ranging from those with neuroregulatory functions to those activated by immune stimuli, thereby providing critical insights into how ribosomes are remodeled.
View details for DOI 10.1101/2023.12.07.570613
View details for PubMedID 38106052
View details for PubMedCentralID PMC10723405